怀牛膝抗着床作用与子宫肥大细胞的关系  

The relationship between anti-implantation of Achyranthes bidentata BI and mast cell in the uterus

作　　者：张文学[1] 杨林松[1] 张新胜[1] 薛德明[1] 张顺利[1] 魏纪东[1] 渠桂荣[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453002 [2]河南师范大学化学与环境工程学院,新乡453002

出　　处：《中国免疫学杂志》2002年第7期492-495,共4页Chinese Journal of Immunology

基　　金：河南省自然科学基金(0111024100);教育厅自然科学基金及重点学科基金资助

摘　　要：目的:探讨怀牛膝苯提取物的抗着床作用与子宫肥大细胞（mast　cell,MC）的关系。方法:孕鼠自孕1d起连续灌眼怀牛膝苯提取物5d（2g/L×2ml/d×5d）;取其孕 3～7d子宫做冰冻切片;用组织化学和免疫组织化学双重染色技术检测MC的数量、胞内TNF-α和表面ICAM-1的表达状况。结果:灌药后,孕3～7d子宫MC总数较对照组显著增多（P<0.001）;INFL-α免疫反应阳性MC（TNF-α+MC）数也显著多于对照鼠（P<0.001）,MC中的TNF-α阳性率（TNF-α+MC/MC总数）由对照组的约10％提高到30％左右;ICAM-1免疫反应阳性MC（ICAM-1+MC）较对照组也显著增加,但ICAM-1阳性率有不同程度下降。结论:怀牛膝可使孕鼠子宫MC数量显著增多、表达TNF-α的能力大幅度提高,这可能是怀牛膝抗着床的免疫学机制之一。Objective:To invetigate the relationship between anti-implantation of Achyranthes bidentata BI and mast cell(MC) in the uterus. Methods: The pregnant mice were treated with benzene extract of Achyranthes bidentata BI from pregnant 1 d(ig 2 g/L× 2 ml/d× 5 d) . The uterus of pregnant 3 - 7 d mice were used to cryostal sections. The doubling staining technique of histochemistry and immunohistochemistry was used to detect the number of MC and the expression of TNF-a and ICAM-1 in MC. Results: Achyranthes bidentata BI could increase apparently the total number of MC and the number of TNF-a positive and ICAM-1 postitive MC in the uterus of pregnant 3 ~ 7 d mice( P <0.001); And TNF-α positive rate also increased obviously but ICAM-1 positive rate decreased. Conclusion: Achyranthes bidentata BI can increase obviously the number of MC and the expression of TNF-α in MC. It could be one of anti-implantation mechanisms of Achyranthes bidentata BI.

关 键 词：抗着床作用 怀牛膝 免疫组织化学 肥大细胞 子宫 

分 类 号：R282.710.5[医药卫生—中药学]