人绒毛膜促性腺激素诱导JEG-3细胞TGF-β_3的表达  

Effect of human chorionic gonadotrophin on TGF-β_3 expression in JEG-3 cell line

作　　者：庞战军[1] 邢福祺[1] 周君桂[2] 

机构地区：[1]第一军医大学南方医院妇产科,广州510515 [2]第一军医大学南方医院惠桥科,广州510515

出　　处：《中国免疫学杂志》2002年第7期496-498,共3页Chinese Journal of Immunology

基　　金：本课题为"973国家重点基础研究发展规划"资助项目;课题编号G1999055903

摘　　要：目的:探讨人绒毛膜促性腺激素（hCG）对绒癌细胞系表达转化生长因子（TGF-β3）的影响。方法:以绒癌细胞来源的JEG-3细胞系为研究对象,采用半定量逆转录多聚酶链反应（RT-PCR）方法,观察HCG对JEG-3细胞表达TGF-β3　mRNA的影响响。结果:分别用0、50、500、5000、25000 mU/ml　hCG处理48h后,JEG-3细胞中TGF-β3　mRNA的含量逐渐被诱导增多,并表现出一定的浓度效应——随hCG作用浓度增高而显著;另外,分别检测受25 000mU/ml hCG处理0、6、12、24、30、36、48h的JEG-3细胞中TGF-β3 mRNA的表达情况,发现TGF-β3表达受hCG的诱导在30h时最强,之后逐渐减弱。结论:hCG可能通过调节TGF-β3在滋养细胞或滋养细胞来源的绒癌细胞中的表达,进而影响细胞的浸润转移。Objective:To explore whether human chorioic gonadotrophin(hCG) might change the invasiveness of trophoblast by regulating the prodution of TGF-β3. Methods: The effect of hCG on TGF-β3 mRNA expression in JEG-3 cells was investigated by employing the semi-quantiative method of reverse transcription polymerase chain reaction(RT-TCR). Results:TGF-β3 was shown to be expressed in JEG-3 cells. After incubated with 0,50,500,5 000,25 000 mU/ml hCG for 48 hours respectively, the expression of TGF-β3 in JEG-3 cells increased gradually with the concentration of hCG increased. Additionally,TGF-β3 mRNA expression in JEG-3 cells incubated with 25 000 mU/ml hCG experienced a significant induction at 30 h point which was followed by a less significant decrease.Conclusion:The autocrine of TGF-β3 might be involved in the effect of hCG on trophoblast or trophoblast-derived choriocarcinoma cell invasiveness.

关 键 词：JEG-3细胞 滋养细胞 肿瘤侵袭 人绒毛膜促性腺激素 转化生长因子 绒癌细胞 

分 类 号：R730.1[医药卫生—肿瘤]