HPLC测定姬松茸中谷胱甘肽的含量  

Determination of Glutathione in Agaricus blazei Murriu by HPLC

作　　者：陈芬[1,2] 赵晓燕[1] 饶钦雄[1] 邢增涛[1] 周昌艳[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]上海海洋大学,上海201306

出　　处：《天然产物研究与开发》2014年第9期1454-1457,共4页Natural Product Research and Development

基　　金：上海市科委国内科技合作项目(11395800500)

摘　　要：本实验建立了HPLC法测定姬松茸中谷胱甘肽的方法，在水提条件下，用Waters RP18色谱柱（4．6mm×250mm，5．0μm），以0．1％三氟乙酸水溶液一甲醇（98：2，v／v）为流动相，流速1．0mL／rain，柱温35℃，检测波长200nm，进样量10μL，谷胱甘肽在8min内出峰。该方法中谷胱甘肽标准品在8—160μo／mL范围内线性良好，R2=0．9997，检出限为0．125μg／mL，加标回收率在94．18％~95．80％之间，RSD〈2％。本方法快速、简便、准确，灵敏度高，适合批量样品的检测与分析。A simple, quick and reliable method was established to determine the content of glutathione （GSH） in Agari- cus blazei Murriu by HPLC. The sample was ultrasonically extracted by distilled water. The HPLC analysis conditions were developed and optimized as follows ：0.1% tritluomacetic acid and methanol （98：2,v/v） were used as mobile pha- ses with the flow rate of 1.0 mL/min, The column oven temperature was set at 35 ℃. The detection wavelength was set at 200 nm. The presented method showed good linear relationship in the range of 8 p.g/mL-160 p.g/mL,R2 =0. 9997, and the detection limit of GSH was 0. 125 μg/mL,the recovery was between 94.18%-95.80% with the RSD less than 2% （n = 9）. The developed HPLC method was suitable for the analysis and determination of samples in large quantity.

关 键 词：谷胱甘肽 高效液相色谱 姬松茸 提取 

分 类 号：TS207.3[轻工技术与工程—食品科学]