胡芦巴中两种芪类成分的纯化工艺研究  

Enrichment of Two Stibenes from Fenugreek(Trigonella foenum-graecum L. ) Seeds

作　　者：何彦峰[1,2] 李聪[3] 索有瑞[1] 王洪伦[1] 

机构地区：[1]中国科学院藏药研究重点实验室 [2]中国科学院大学,北京100049 [3]青海大学生态环境工程学院,西宁810001

出　　处：《天然产物研究与开发》2014年第9期1497-1502,1453,共7页Natural Product Research and Development

基　　金：中国科学院生命科学领域优秀青年科技专项项目(KSCX2-EW-Q-23);国家自然科学基金项目(81001698)

摘　　要：本文运用高效液相色谱(HPLC)含量分析方法,以胡芦巴种子中土大黄苷和丹叶大黄素为目标化合物,以其总回收率和总含量为指标,采用单因素和响应面分析法,优化了大孔吸附树脂纯化胡芦巴中这两种芪类成分的工艺条件和参数。结果表明,在11种大孔吸附树脂中,HPD300型树脂的纯化效果最佳,最佳纯化工艺条件为:以25.0 mL、pH 5.0的胡芦巴种子粗提液上柱,流速0.5 mL/min,充分吸附后用3 BV去离子水洗柱,然后用27.0 mL 50%乙醇溶液以流速0.6 mL/min进行解吸。此工艺的平均回收率为91.76%;经HPD300树脂纯化后提取物中芪类成分(包括土大黄苷和丹叶大黄素)总含量从15.5%提高到56.94%。In this study, macroporons resin column chromatography （MRCC） was used for the enrichment of two main stibenes, namely rhaponticin and rhapontigenin,in fenugreek （ Trigonellafoenum-graecum L. ） seeds. The MRCC enrich- ment conditions were optimized using single-factor tests and response surface methodology （RSM） while the total recov- ery and the total content of two compounds were recorded as response index. The content of targeted compounds was de- termined by HPLC. Finally, HPD300 macroporous resin was selected from 11 resins. The optimized enrichment conditions were as follows：25.0 mL as sample size ,5.0 as pH value of sample solution,O. 5 ml_/min as flow rate of sample solu- tion. After fully adsorption,3 BV deionized water was used to wash out the substance without being absorbed,and then 27.0 mL of ethanol （50%） was used for the desorption of targeted compounds, and the flow rate was set at 0.6 mL/ rain. Under these optimized parameters, the total recovery of two compounds was over 91.76%, and the total concentra- tions of two compounds enriched by HPD300 macroporeus resin increased from 15.5 to 56.94%.

关 键 词：胡芦巴 芪类成分 大孔吸附树脂 高效液相色谱 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]