Krüpple样转录因子6和核组蛋白-2在大鼠胃癌模型胃肠道组织中的表达  

Expression of Kruppel-like factor 6 and nucleobindin-2 genes in gastrointestinal tract of rats with gastric cancer

作　　者：王建锋[1] 王耿泽[1] 宋展[1] 厉冰[1] 

机构地区：[1]河南省南阳市中心医院普外科,473000

出　　处：《中华实验外科杂志》2014年第9期1944-1946,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察在胃癌大鼠模型胃肠道组织中Kr＆#252;pple样转录因子6（Klf-6）和核组蛋白-2（NUCB2）及相关基因表达的影响.方法 将Wister大鼠96只分为3组,A组32只,采用黄曲霉素B1（AFB1）对大鼠进行诱导处理3个月,制备胃癌大鼠模型.B组32只,应用AFB1对大鼠进行诱导处理6个月,制备胃癌大鼠模型.C组32只,为正常对照组.用免疫组织化学法检测3组大鼠胃肠道组织中NUCB2和Klf-6的表达水平.逆转录-聚合酶链反应（RT-PCR）技术检测两组大鼠胃肠道组织中NUCB2和Klf-6基因mRNA表达水平,比较其差异.结果 A、B组成功制备了胃癌大鼠模型,但不同诱导时间两组大鼠胃癌发病的程度和发病率不同,差异有统计学意义（P＜0.05）.Klf-6和NUCB2在B组的表达率高于A组和C组,差异有统计学意义,A组Klf-6和NUCB2表达又高于C组,差异有统计学意义（x2 =3.17、4.95、3.79,P＜0.05）.RT-PCR结果显示,B组胃肠道组织中Klf-6和NUCB2基因的mRNA表达水平高于A组,A组高于C组,差异有统计学意义（x2=2.98、4.13、3.52,P＜0.05）.结论 肌氨酸乙酯盐酸盐诱导胃癌模型时间越长,胃癌大鼠胃肠道Klf-6和NUCB2的表达也越强.Objective To discuss the expression of Kr＆#252;ppel-like factor 6 （Klf-6） and nucleo-bindin-2 （NUCB2） genes in gastrointestinal tract of rats with gastric cancer.Methods Ninety-six adult Wister rats were randomly divided into three groups：32 rats each in groups A and B were drenched intraperitoneally with sarcosine ethyl ester hydrochloride to induce gastric cancer for 3 and 6 months respectively,and 32 rats in group C were injected with normal saline.The gastrointestinal tract was examined by electronic scale at 3rd and 6th month after modeling.The Klf-6 and NUCB2 expression in gastrointestinal tract of rats was observed,and the mRNA levels were evaluated by reverse transcription-polymerase chain reaction （RT-PCR） after the rats were decapitated.The statistical analysis was done by using SPSS 14.0 statistical software.Results All the 96 rats were involved in the analysis of results.Positive rate of Klf-6 and NUCB2 in group A was higher than in groups B and C,and that in group B higher than in group C （x2 =3.17,4.95,and 3.79,P ＜0.05）.RT-PCR revealed that the expression levels of Klf-6 and NUCB2 mRNA in group A were higher than in groups B and C,and those in group B higher than in group C （x2 =2.98,4.13 and 3.52,P ＜ 0.05）.Conclusion Combined detection of the expression of Klf-6 and NUCB2 in gastric cancer may give some guideline for the clinical diagnosis,illness evaluation,treatment and prognosis of gastric cancer.

关 键 词：胃癌 Krupple样转录因子6 核组蛋白-2 

分 类 号：R735.2[医药卫生—肿瘤]