富含半胱氨酸的酸性分泌蛋白对胰腺癌细胞增殖、迁移和侵袭能力的影响  

Effects of secreted protein acidic and rich in cysteine on proliferation, migration and invasion ability of pancreatic cancer cells

作　　者：孙烈[1] 陈国卫[1] 尹杰[1] 朱静[1] 田孝东[1] 杨尹默[1] 万远廉[1] 刘玉村[1] 

机构地区：[1]北京大学第一医院普通外科, 100034

出　　处：《中华实验外科杂志》2014年第9期1954-1956,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察外源性富含半胱氨酸的酸性分泌蛋白（SPARC）对胰腺癌细胞增殖、迁移和侵袭力等生物学行为的影响.方法 将胰腺癌细胞Mia PaCa-2和PANC-1中加入不同浓度的外源性SPARC（0、0.5、1.0、3.0、5.0、10.0 mg/L）作为实验组,以空白组作对照,采用噻唑蓝（MTT）实验分析胰腺癌细胞增殖能力的变化;将胰腺癌细胞Mia PaCa-2和PANC-1中加入5 mg/L外源性SPARC,以空白组为对照,培养24 h,采用单个细胞移动实验分析胰腺癌细胞迁移能力的变化,采用Matrigel侵袭力实验分析胰腺癌细胞侵袭能力的变化.结果 外源性SPARC能够抑制胰腺癌细胞的增殖,且抑制作用具有浓度依赖性,10.0 mg/L外源性SPARC可使Mia PaCa-2和PANC-1细胞的增殖能力分别下降（32.8±7.4）％和（32.5±1.8）％.5.0 mg/L外源性SPARC能够使Mia PaCa-2和PANC-1细胞在24 h的移动距离分别下降26％和39％,细胞侵袭转移数目分别下降30％和56％.结论 外源性SPARC可抑制胰腺癌细胞的增殖、迁移和侵袭能力,细胞外基质成分对恶性肿瘤的生物学行为可产生影响.Objective To investigate the effects of exogenous secreted protein acidic and rich in cysteine （SPARC） on proliferation,migration and invasion ability of human pancreatic cancer cell lines.Methods The cuhured Mia PaCa-2 and PANC-1 pancreatic cancer cells were established in the presence of different concentrations （0,0.5,1.0,3.0,5.0,10.0 mg/L） or absence of exogenous SPARC as experimental and control groups.The change of proliferation was measured by methyl thiazol tetrazolium （MTT） assay.The 24 h cultured Mia PaCa-2 and PANC-1 pancreatic cancer cells were established in the presence （5 mg/L） or absence of exogenous SPARC as experimental and control groups,and the change of migration ability was detected by single cell migration assay and the change of invasion ability by Matrigel invasion assay respectively.Results Exogenous SPARC could inhibit the proliferation of cells in a concentration-dependent manner.Cell proliferation was inhibited in the presence of 10.0 mg/L exogenous SPARC by （32.8 ± 7.4） % and （32.5 ± 1.8） % in Mia PaCa-2 and PANC-1,respectively.5.0 mg/L SPARC reduced the distance covered within 24 h by 26% and 39%,and in the presence of 5.0 mg/L SPARC the number of invasive cells was inhibited by 30% and 56% in 24 h,respectively.Conclusion Exogenous SPARC could inhibit the proliferation,migration and invasion ability in pancreatic cancer cells,suggesting extracelluar matrix may affect biological functions of malignant tumors.

关 键 词：富含半胱氨酸的酸性分泌蛋白 细胞外基质 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]