CD13在骨瘤及骨肉瘤中的表达及意义  

Expression and significance of CD13 in osteoma and osteosarcoma

作　　者：陈文革[1] 杨朝晖[1] 刘清高[1] 鄢含坤 

机构地区：[1]湖北民族学院附属民大医院骨外科, 湖北恩施445000

出　　处：《中华实验外科杂志》2014年第9期2063-2064,共2页Chinese Journal of Experimental Surgery

摘　　要：目的 观察CD13在骨瘤及骨肉瘤中的表达及意义.方法 免疫组织化学法检测20例良性骨瘤及56例骨肉瘤组织中CD13蛋白的表达,逆转录-聚合酶链反应（RT-PCR）检测CD13mRNA在20例骨肉瘤及相应肉瘤旁组织中的表达,分析CD13表达与临床病理参数的关系.结果 免疫组织化学结果显示CD13蛋白在良性骨瘤及骨肉瘤组织中的阳性表达率分别为64.3％、10.0％,差异有统计学意义（P＜0.05）.CD13蛋白在Ⅲ期及Ⅰ～Ⅱ期骨肉瘤组织中的阳性表达率分别为90.0％、58.9％,差异有统计学意义（P＜0.05）,而与患者的年龄、性别、肿瘤发生部位、肿瘤大小无明显相关（P＞0.05）.RT-PCR结果显示CD13 mRNA在肉瘤中的表达明显高于肉瘤旁组织（P＜0.05）.结论 CD13的表达与骨肉瘤发生及进展明显相关.Objective To investigate the expression and significance of CD13 in osteoma and osteosarcoma.Methods Immunohistochemistry was used to detect the protein expression of CD13 in 20 cases of benign osteoma and 56 cases of osteosarcoma.reverse transcription-polymerase chain reactionn （RT-PCR） method was used to examine the level of CD13 mRNA in 20 cases of osteosarcoma and corresponding adjacent tissue,and the relationship between expression of CD13 and clinicopathological parameters was evaluated.Results Immunohistochemistry showed the positive rate of CD13 protein in benign osteoma and osteosarcoma tissues was 64.3％ and 10.0％ respectively （P ＜ 0.05）.CD13 protein in osteosarcoma tissues of phase Ⅲ and phase Ⅰ-Ⅱ was 90.0％ and 58.9％ respectively （P ＜ 0.05）.The CD13 protein expression was not correlated with age,sex,tumor location,and tumor size （P ＞ 0.05）.RT-PCR results showed CD13 mRNA expression level was significantly higher in sarcoma than that in the adjacent tissue （P ＜0.05）.Conclusion CD13 is obviously correlated with the occurrence and progression of osteosarcoma.

关 键 词：骨肉瘤 CD13 免疫组织化学 

分 类 号：R738.1[医药卫生—肿瘤]