七彩红竹查尔酮合成酶IhCHS1基因的克隆与分析  

Cloning and Sequence Analysis of Chalcone Synthase Gene IhCHS1 from Indosasa hispida cv. rainbow

作　　者：周旭[1,2] 王晨晨[1,2] 毕玮[2,3] 王娟[2,3] 杨宇明[3] 王毅[2,3] 

机构地区：[1]西南林业大学林学院,云南昆明650224 [2]云南林业科学院国家林业局云南珍稀濒特森林植物繁育和保护重点实验室,云南昆明650201 [3]云南林业科学院云南省森林植物培育与开发利用重点实验室,云南昆明650201

出　　处：《竹子研究汇刊》2014年第3期11-16,共6页Journal of Bamboo Research

基　　金：云南省中青年学术技术带头人后备人才培养项目(2010CI016);云南省基础研究重点项目(2013FA054);国家林业局云南珍稀濒特森林植物繁育和保护重点实验室开发基金共同资助

摘　　要：通过已报道查尔酮合成酶基因的保守序列设计特异引物,扩增得到七彩红竹(Indosasa hispida cv.rainbow)查尔酮合成酶(Chalcone Synthase,CHS)基因片段,再以RACE法获得CHS基因全长cDNA序列。该cDNA全长1953 bp,含1542 bp的开放阅读框,编码含513个氨基酸残基的蛋白质。多序列比对结果表明IhCHS1推断的蛋白与节节麦(Aegilops tauschii)等禾本科植物的CHS相似性在87%以上,临位法构建系统发生树显示IhCHS1与禾本科植物CHS亲缘关系较近。RT-PCR结果显示IhCHS1在幼嫩红秆中大量表达。A cDNA encoding chalcone synthase（IhCHS1） was isolated from the red culm of Indosasa hispida cv.rainbow by rapid amplification of cDNA ends （RACE）,and the specific primers were designed by the reported CHS conserved sequence.The full-length cDNA of IhCHS1 was 1953 bp with a 1542 bp open reading frame （ORF） that corresponded to a predicted protein of 513 amino acid deduced protein.Multiple sequence alignment indicated that the IhCHS1 sequence was highly conserved and shared high sequence identity （＞ 87％） with chalcone synthases from other Poaceae plants.The phylogenetic analysis showed a close genetic relationship between IhCHS1 and CHS from other Poaceae species.Gene expression analysis via RT-PCR showed that IhCHS1 was most highly expressed in the young culm.

关 键 词：七彩红竹 查尔酮合成酶 基因克隆 表达分析 

分 类 号：S795[农业科学—林木遗传育种] Q943.2[农业科学—林学]