肝细胞癌CT动态增强密度与肿瘤分化程度、CD34、CK19、GPC3、P53、Ki67表达的多因素相关性分析  

The multivariate regression analysis on the correlation of CT dynamic enhancement density with histologic differentiation and expressions of CD34,CK19,GPC3,P53,Ki67 in hepatocellular carcinoma patients

作　　者：崔雄伟[1] 郑加生[1] 钱智玲[1] 张世杰[2] 崔石昌[1] 

机构地区：[1]首都医科大学附属北京佑安医院微创介入科,100069 [2]首都医科大学附属北京佑安医院病理中心,100069

出　　处：《北京医学》2014年第9期724-727,共4页Beijing Medical Journal

基　　金：国家科技支撑计划(2012BAI15B08);北京佑安医院肝病艾滋病科研基金(BJYAH-2011-034)

摘　　要：目的探讨肝细胞癌(HCC)螺旋CT多期扫描动脉期、门脉期强化值与肿瘤分化程度、CD34、CK19、GPC3、P53、Ki67表达的相关性。方法 67例经病理证实的HCC患者进行CT多期扫描,分别计算67个癌灶的动脉期强化值和门脉期强化值。肝穿刺组织进行HE染色和免疫组化法检测,评定肿瘤分化程度、CK19、CD34、GPC3、P53、Ki67表达强度。对HCC动脉期、门脉期强化值与上述细胞因子表达关系进行多元线性回归分析,筛选、建立理想的多元线性回归方程。结果 67个癌灶呈"快进快出"表现,符合典型的HCC CT表现特征。HCC动脉期强化值为(38.9±13.1)HU;多元线性回归方程为:动脉期强化值=27.381-10.0161×CK19+4.245×CD34+3.503×GPC3。HCC门脉期强化值为(44.7±11.7)HU;多元线性回归方程为:门脉期强化值=26.869+8.809×CK19+4.800×CD34+3.610×GPC3。结论多期增强扫描可反映多种细胞因子的表达、HCC的生物学特性。Objective Objective To study the relationship between the difference contrast enhancement density in arterialphase and portalphase on multiphase spiral CT with histologic differentiation and expressions of CK19,CD34,GPC3,P53,Ki67 in hepatocellular carcinoma（HCC） patients, and to explore the biological features of HCC by multiphase spiral CT. Methods Neoplastic foci from 67 patients with HCC confirmed histopathologically were studied.Multiphase spiral CT scans was performed. The difference contrast enhancement density in arterialphase and portalphase were calculated. The histologic differentiation was estimated by HE staining in liver biopsy samples. The expressions of CK19,CD34,GPC3,P53 and Ki67 were detected by immunohistochemical methods. The multiple linear regression equation was established with the difference contrast enhancement density in arterialphase and portalphase,the histologic differentiation and the expressions of cytokines. Results Sixty-seven neoplastic foci performed typical CT feature of HCC with ＂contrast enhancement density in arterialphase and wash-out in portalphase＂. The difference contrast enhancement density in arterialphase was（38.9 ±13.1）HU. The multiple linear regression equation showed the difference contrast enhancement density in arterialphase=27.381-10.0161 ×CK19 ＋4.245 ×CD34 ＋3.503 ×GPC3. The difference contrast enhancement density in portalphase was（44.7 ±11.7）HU. The multiple linear regression equation showed the difference contrast enhancement density in portalphase=26.869＋8.809×CK19＋4.800×CD34＋ 3.610×GPC3. Conclusion Multiphase spiral CT is a method to reflect the expressions of various cytokines for assessing biological behaviors of HCC.

关 键 词：肝细胞癌 CT 动脉期 门脉期 分化 细胞因子 

分 类 号：R735.7[医药卫生—肿瘤]