雌激素受体α基因RFLP与脑动脉瘤的相关性  

Relationship between RFLP of the ERα gene and intracranial aneurysm

作　　者：伍刚[1] 刘策[1] 周敬安[1] 许百男[2] 

机构地区：[1]解放军第309医院神经外科,北京100091 [2]解放军第301医院神经外科,北京100853

出　　处：《现代医学》2014年第8期884-887,共4页Modern Medical Journal

摘　　要：目的:研究脑动脉瘤与雌激素受体(estrogen receptorα,ERα)基因PvuⅡ和XbaⅠ限制性片段长度多态性(restriction fragment length polymorphism,RFLP)的关系。方法:采集2006年2月至2007年4月解放军总医院神经外科患者血样标本,其中脑动脉瘤84例,非动脉瘤88例。抽提基因组DNA,扩增ERα第1内含子基因片段。扩增片段分别进行PvuⅡ和XbaⅠRFLP分析。结果:扩增片段长度为1.3 kb。PvuⅡRFLP结果显示,对照组PP占14.8%,Pp占48.9%,pp占36.3%;脑动脉瘤组PP占22.6%,Pp占58.3%,pp占19.1%。对照组与脑动脉瘤组之间PvuⅡRFLP差异有统计学意义(χ2=6.760,P<0.05)。XbaⅠRFLP结果显示,对照组XX占4.6%,Xx占35.2%,xx占60.2%;脑动脉瘤组XX占2.4%,Xx占35.7%,xx占61.9%。两组之间差异无统计学意义(χ2=0.600,P>0.05)。结论:ERα第1内含子PvuⅡ限制内切片段长度多态性与脑动脉瘤破裂有关。Objective： To investigate the relationship between gene restriction fragment length polymorphism（RFLP） of ERα and intracranial aneurysm. Methods： 84 patients with intracranial aneurysm and 88 patients with non-aneurysm were collected from February,2006 to April,2007. The blood samples were harvested which were used to extract the genome DNA. The augment fragments were incised by PvuⅡ and XbaⅠ. Results： The augment fragment length was 1. 3 kb. The RFLP of control group PvuⅡ was PP 14. 8%,Pp 48. 9% and pp 36. 3%,while the RFLP of aneurysm group Pvu Ⅱ was PP 22. 6%,Pp 58. 3% and pp 19. 1%. It was significant difference between aneurysm group and control group（ χ2= 6. 760,P 〈 0. 05）. The RFLP of control group XbaⅠ was XX4. 6%,Xx 35. 2% and xx 60. 2%. The RFLP of aneurysm group XbaⅠ was XX 2. 4%,Xx 35. 7% and xx 61. 9%.There was no significant difference（χ2= 0. 600,P 〉 0. 05）. Conclusion： The RFLP of ERα first intron PvuⅡ gene polymorphism is related to intracranial aneurysm.

关 键 词：脑动脉瘤 雌激素受体 基因多态性 

分 类 号：R739.41[医药卫生—肿瘤]