不同地域人群血清代谢谱分析  

Serum metabolic profiles among different geographical populations

作　　者：曹瑞丽[1,2] 郭静利[3] 孙博[1] 张琪[1] 杨征[3] 颜贤忠[1] 

机构地区：[1]国家生物医学分析中心,北京100850 [2]解放军264医院药剂科,太原030001 [3]军事医学科学院基础医学研究所,北京100850

出　　处：《军事医学》2014年第8期633-637,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81001419)

摘　　要：目的探讨地域差异对人体血清代谢谱的影响。方法采集来自我国9个省份的血清样本,共计169例,按照地理位置的归属,将样本划分为东西南北4个区域。采用核磁共振(NMR)的代谢组学技术与多元统计方法相结合,对血清NMR谱图进行分段积分、归一化处理,所得数据输入到多元统计分析软件SIMCA-P 10.0,经正交信号校正(OSC)后采用偏最小二乘法(PLS)进行模式识别,寻找东西部人群、南北方人群血清中具有差异的代谢物。结果东西部、南北方健康人群的血清代谢谱中,葡萄糖、脂蛋白、氨基酸、酮体、肌酸、胆碱等多种小分子代谢产物浓度存在差异。结论由多种因素(气温、降水、民俗文化、生活习惯等)造成的地域差异会导致人体血清代谢谱的差异,可以对流行病学及营养代谢组学的研究提供相应的参考。Objective To investigate the effect of geographical differences on human serum metabolic profiles .Methods A total of 169 serum samples were collected from healthy individuals from 9 provinces in China and divided into four groups according to the geographical location .The nuclear magnetic resonance （ NMR） data of these samples were segmentally inte-grated, normalized,and analyzed with multivariate statistical methods using SIMCA-P 10.0 software to investigate the differ-ence in serum metabolites between eastern and western populations or between northern and southern populations .Results There were differences in a variety of small molecule metabolites in serum between eastern and western populations or be -tween northern and southern,including glucose,lipoproteins,amino acids,ketones,creatine and choline.Conclusion The geographical differences in a variety of factors （temperature,precipitation,folk culture, living habits,etc） result in the differences in human serum metabolic profiles ,which can provide appropriate reference for epidemiological studies and nu -tritional metabonomics .

关 键 词：代谢组学 血清 核磁共振 地域差异 营养 

分 类 号：R331[医药卫生—人体生理学]