蜕皮甾酮对2型糖尿病大鼠肝细胞IRS-2蛋白表达的影响  

Effect of ecdysterone on expression of insulin receptor substrate 2 in liver cell of type 2 diabetic rats

作　　者：朱玉霞[1] 孙丽莎[1] 杨矫[1] 陈秋[1] 

机构地区：[1]成都中医药大学附属医院,成都610075

出　　处：《山东医药》2014年第33期10-12,共3页Shandong Medical Journal

基　　金：教育部新世纪优秀人才支持计划(NCET-08-0903);四川省国际合作项目(2008HH0024)

摘　　要：目的探讨蜕皮甾酮对2型糖尿病大鼠肝细胞胰岛素受体底物2(IRS-2)蛋白表达的影响。方法选择42只大鼠,14只作为正常组,常规饲料喂养,余下28只先以高脂喂养加小剂量STZ注射诱导2型糖尿病大鼠模型,造模成功后,随机分为模型组和治疗组,分别给予高脂饲料喂养、高脂饲料喂养+蜕皮甾酮灌胃治疗。5周后取大鼠肝组织标本,应用免疫组化和RT-PCR技术检测IRS-2蛋白和mRNA含量。结果正常组和治疗组肝细胞IRS-2蛋白及mRNA含量均明显高于模型组(P<0.05或<0.01)。结论蜕皮甾酮可提高2型糖尿病大鼠肝细胞IRS-2蛋白和mRNA含量,这可能是蜕皮甾酮改善2型糖尿病大鼠胰岛素抵抗的机制之一。Objective To investigate the effect of ecdysterone on the expression of insulin receptor substrate 2 （ IRS-2 ） in liver cell of type 2 diabetic rats .Methods 14 of the 42 rats were randomly selected to the normal control group , and was given ordinary rat food .The other 28 rats were made into type 2 diabetes mellitus rat models by using the high fatty diet and small dose of SIZ , and then diveided into model group and treatment group .The rats in model group or treatment group was given high fatty diet or high fatty diet fecdysterone .IRS-2 protein and mRNA in liver tissues of rats were detected by immunohistochemistry assay and RT-PCR assay after five weeks .Results The expression of IRS-2 protein and mRNA in normal group and treatment group were higher than those of the model group （P〈0.01or 〈0.01）.Conclusions Ecdyste-rone led to up-regulation expression of IRS-2 protein and IRS-2 mRNA of liver tissue in diabetic rats .This might play a role in ecdysterone-improved hepatic insulin resistance .

关 键 词：蜕皮甾酮 2型糖尿病 胰岛素受体底物2 胰岛素抵抗 大鼠 INSULIN receptor substrate 2 

分 类 号：R587.1[医药卫生—内分泌]