北极海洋沉积物中放线菌多样性及抑菌活性筛选  

Diversity and Antibacterial Activity Screening of Actinomycetes in the Marine Sediments of North Pole

作　　者：常显波[1] 柳瑞翠[1] 刘文正[2] 张晓华[2] 

机构地区：[1]烟台大学环境与材料工程学院,山东烟台264005 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《湖北农业科学》2014年第13期3014-3018,共5页Hubei Agricultural Sciences

基　　金：国家高技术研究发展计划项目(2007AA09Z434);烟台大学博士启动基金项目(HJ11B26);国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金项目(MBSMAT-2012-01)

摘　　要：采用平板涂布法利用6种培养基从北极海洋沉积物中共分离出109株放线菌。选取34株代表菌株进行16S rDNA测序分析。结果表明,它们分属于链霉菌属(Streptomyces)、假诺卡氏菌属(Pseudonocardia)和拟诺卡氏菌属(Nocardiopsis),其中Streptomyces为优势菌属;不同采样点获得放线菌的种类和数量有较大不同,站位点P37分离到的放线菌数量和种类最多;在109株放线菌中,有39株对病原菌有抑菌活性,其中有21株、11株和17株放线菌分别对枯草芽孢杆菌、大肠杆菌和金黄色葡萄球菌生长有抑制作用。Diversity and actinobacterial diversity in the sediments of North Pole was investigated by selective isolation method.109 actinomycetes were isolated using six different culture media （M1-M6）.Bioinformatics analysis of the 16S rDNA sequences assigned the 34 representative isolates to three genera （Streptomyces,Pseudonocardia and Nocardiopsis）,and Streptomyces was the dominant genus.The type of culture medium exhibited significantly the number and diversity of actinomycetes recovered.Out of 109 isolates,39 isolates exhibited antimicrobial activity against human pathogens.The proportion of actinomycetes with antibacterial activity was highest from the culture medium M5.

关 键 词：北极 海洋沉积物 放线菌 多样性 抑菌活性 

分 类 号：Q939.99[生物学—微生物学]