hTERT启动子调控的PUMA腺病毒转染卵巢癌COC1细胞的研究  

Recombinant adenovirus containing PUMA gene regulated by human telomerase reverse transcriptase promoter transfected into ovarian cancer COC1 cells

作　　者：李雨聪[1] 王冬[1] 

机构地区：[1]重庆市肿瘤研究所妇瘤科,400030

出　　处：《重庆医学》2014年第26期3452-3454,共3页Chongqing medicine

基　　金：重庆市卫生局课题(2011-2-366)

摘　　要：目的探讨卵巢癌COC1细胞中大量表达p53正向凋亡调控因子(PUMA)在抑制COC1细胞中的作用。方法采用人端粒酶逆转录酶(hTERT)启动子调控的PUMA基因腺病毒载体(Ad-hTERT-PUMA)转染卵巢癌COC1细胞,通过细胞活性检测试剂盒和凋亡试剂盒研究COC1细胞的生长情况,同时分析表达PUMA的水平。结果转染Ad-hTERT-PUMA后,PUMA大量表达,抑制COC1细胞生长,促进细胞凋亡。结论 Ad-hTERT-PUMA成功转染卵巢癌COC1细胞并大量表达,起到了抑制卵巢癌细胞增长的作用。Objective To over-express the P53 upregulated modulator of apoptosis（PUMA） in ovarian cancer COC1 cells for playing the role to restrain the cell growth .Methods Adenovirus vector （Ad）-human telomerase reverse transcriptase promoter （hTERT）-PUMA was transfected into ovarian cancer COC1 cells .Then the growth of COC1 cells was studied by CCK-8 and the apoptosis kit and the level of PUMA was analyzed .Results After transfection of Ad-hTERT-PUMA ,PUMA was over-expressed in COC1 cells ,which restrained the cells growth and promoted the cell apoptosis .Conclusion Ad-hTERT-PUMA is successfully transfected into ovarian cancer COC1 cells and PUMA is over-expressed to play the role for restraining the cells growth .

关 键 词：端粒  末端转移酶 p53正向凋亡调控因子 卵巢肿瘤 腺病毒科 

分 类 号：R737.31[医药卫生—肿瘤]