当归对吊尾大鼠比目鱼肌收缩功能的影响  

Angelica and the contractile properties of soleus muscle in tail-suspended rats

作　　者：杜蓓[1] 高云芳[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2014年第4期588-594,共7页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(31270455);2011年度高等学校博士学科点专项科研基金资助课题(20116101110013);陕西省国际科技合作与交流计划基金资助项目(2013KW26-01)

摘　　要：探讨当归对吊尾14天大鼠比目鱼肌(SOL)收缩功能的影响及可能的作用机制。以尾部悬吊法建立大鼠废用性肌萎缩模型,将24只雌性SD大鼠分为正常组(CON),吊尾组(TS)和灌胃当归组(TS+Ang)。用PowerLab系统测定比目鱼肌等长收缩与强直收缩最大张力;定磷法测定Ca2+-ATP酶活性。结果显示:1 TS组肌肉湿重(MWW)和肌肉湿重/体重(MWW/BW)比CON组显著降低,TS+Ang组肌肉湿重升高了17%(P<0.05),而肌肉湿重/体重不具统计学意义;2 TS组等长收缩最大张力(Pt)和强直收缩最大张力(Po)比CON组均显著降低;与TS组相比,TS+Ang组Pt值和Po值分别增加91%和49%(P<0.01);3与CON组相比,TS组等长收缩最大张力达到一半的时间(TP50),达到等长收缩最大张力的时间(TPT)以及从最大张力峰值到舒张50%(RT50)和75%(RT75)的时间均显著缩短;与TS组相比,灌胃当归组分别增加109%,51%,57%和76%(P<0.01);4吊尾组肌浆网Ca2+-ATP酶活性显著升高,灌胃当归后,Ca2+-ATP酶活性降低10%(P<0.05)。结果说明:当归能有效缓解吊尾造成的肌肉收缩张力的下降和时程的缩短,当归对抗吊尾引起的比目鱼肌Ca2+-ATP酶活性的升高可能是其抗废用性肌萎缩的重要机制之一。To investigate the effects of angelica on soleus function in 14-day tail-suspended rats.The disuse atrophy model is established for tail-suspended female Sprague-Dawley rats.The rats were randomly divided into control group （CON）,tail-suspended with water （TS） group and tail-suspended with angelica （TS ＋ Ang） group.Contraction tension and time were determined with PowerLab system （SEN.3301,Japan）.Sarcoplasmic reticulum Ca2＋-ATPase activity was measured with the Phosphate Determination method.① Compared with the CON group,both the MWW and MWW/BW of soleus decreased significantly （P ＜ 0.01） in the TS group.In TS ＋ Ang group,MWW increased by 17％ （P ＜ 0.05）,and MWW/BW has no statistical significance; ② Both the peak twitch tension （Pt） and peak tetanic tension （Po） of soleus decreased significantly （P ＜0.01）in the TS group.The Pt and Po increased by 91％ and 49％ in the TS ＋ Ang group （P ＜ 0.01）; ③The time to 50％ peak tension （TP50） and time to peak tension （TPT） decreased （P ＜0.01） in the TS group.TP50 and TPT increased by 109％ and 51％ with Ang （P ＜0.01）.The time from peak tension to 50％ relaxation （RT50） and time from peak tension to 75％ relaxation （RT75） decreased in the TS group.RT50 and RT75 rose by 57％ and 76％ in the TS ＋ Ang group （P ＜ 0.01） ; ④ Ca2 ＋-ATPase activity of TS group increased.After intragastric administration of angelica,Ca2＋-ATPase activity decreased by 10％ （P ＜ 0.05）.Angelica may alleviate the decrease of muscle contractile force and increase of shortening velocity in atrophied soleus,possibly by means of decreasing sarcoplasmic reticulum Ca2＋-ATPase activity.

关 键 词：肌萎缩 等长收缩 强直收缩 比目鱼肌 Ca2-ATP酶 

分 类 号：R258[医药卫生—中医内科学]