光辉霉素A对人胃癌BGC823细胞增殖、凋亡、迁移的影响及其机制  

Effects and its mechanism of mithramycin A on proliferation,apoptosis and migration of human gastric carcinoma BGC823 cell

作　　者：马西强 刘庆彤[2] 谢江柳 王永翔[1] 王洋[1] 陈永华[2] 师水生[2] 

机构地区：[1]山西医科大学,山西太原030000 [2]山西医科大学第二医院消化科,山西太原030001

出　　处：《中国临床研究》2014年第9期1049-1052,共4页Chinese Journal of Clinical Research

摘　　要：目的观察光辉霉素A(MIT)对人低分化胃癌BGC823细胞增殖、凋亡、迁移的作用,以及对转录因子Sp1和乙酰肝素酶(heparanase,Hpa)表达的影响,并探讨其可能的机制。方法采用不同浓度的MIT作用于胃癌BGC823细胞,以Cell Counting Kit-8(CCK8)法测定对细胞增殖的影响;以流式细胞仪测定对细胞凋亡的影响;以细胞划痕法观察对细胞迁移能力的影响;以实时荧光定量聚合酶链反应(Real-time PCR)法定量测定细胞内Sp1和Hpa mRNA表达的变化。结果 MIT抑制胃癌BGC823细胞增殖的效应呈浓度和时间依赖性(P均<0.05);不同浓度(0、0.15、0.30、0.60μmol/L)的MIT作用于胃癌BGC823细胞24 h后,细胞的凋亡率随药物浓度的增加而增加,分别为(2.100±0.985)、(13.533±0.777)、(21.533±0.874)、(27.500±0.964)%,即MIT促进胃癌BGC823细胞的凋亡呈浓度依赖性(P<0.05);MIT对胃癌BGC823细胞迁移的抑制作用具有量效和时效性;胃癌BGC823细胞Sp1和Hpa mRNA表达量随MIT浓度的增加而降低(P均<0.05)。结论 MIT对胃癌BGC823细胞增殖、凋亡、迁移影响可能通过抑制Sp1、Hpa的表达而实现。Objective To observe the effects of mithramycin A （MIT）on the proliferation, apoptosis and migration of human poorly differentiated gastric cancer BGC823 cell and on expression of transcription factor Spl and heparanase（Hpa） and explore its potential mechanism. Methods BGC823 ceils were treated with different concentrations of MIT. The proliferation effect on BGC823 cell was detected by Cell Counting Kit-8 （ CCK8 ） assay ; cell apoptosis effect was measured by flow cytometry;migration effect was observed by scratch cells assay;Spl and Hap mRNA expressions were analyzed by real- time fluorescence quantitative polymerase chain reaction （ Real-time PCR） method, Results MIT inhibited proliferation of BGC823 ceils with concentration-and time-dependent manner （ all P 〈 0.05 ）. After different concentrations [ （0,0. 15, 0. 30 and 0.60） μmol/L） ] of MIT acted to BGC823 cell for 24 hours,the cell apoptosis rate increased with the increase of MIT concentration [ （2. 100 ± 0. 985）%, （ 13. 533 ± 0. 777）%, （21. 533 ± 0. 874）% and （27. 500± 0. 964）%, respectively） ] ,namely MIT promoted BGC823 cell apoptosis with concentration-dependent manner （P 〈 0. 05 ）. The inhabiting effect of MIT on migration ability had dose-effect and time-effect. The expression quantities of Spl and Hpa mRNA in BGC823 cells decreased with the increase of MIT （ all P 〈 0. 05 ）. Conclusions The effects of MIT on proliferation, apoptosis and migration of gastric cancer BGC823 cells might be achieved via inhabiting the Spl and Hpa expressions.

关 键 词：胃肿瘤 光辉霉素A 转录因子SP1 乙酰肝素酶 增殖 凋亡 迁移 转移 

分 类 号：R735.2[医药卫生—肿瘤]