根管冲洗液对多物种细菌生物膜的影响  

Effect of Root Canal Irrigation Solution on Multispecies Biofilms

作　　者：逄爱慧[1] 李明[1] 杜田丰[1] 杨焰[1] 万盼[1] 甘艳[1] 马净植[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,武汉430030

出　　处：《华中科技大学学报（医学版）》2014年第4期437-440,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨洗必泰葡萄糖酸盐(CHX)作为根管冲洗试剂,对多物种细菌生物膜的影响。方法在Ⅰ型胶原包被的羟磷灰石片上制备多物种细菌生物膜,应用2%CHX冲洗,处理3min后,激光扫描共聚焦显微镜观察灭菌效果。结果经过有氧或厌氧培养3周的多物种细菌生物膜生长状态良好;应用2%CHX处理有氧培养或厌氧培养多物种细菌生物膜可以杀灭一部分细菌,死菌比例分别为(33.0±3.0)%、(40.0±2.8)%,无菌去离子水几乎没有杀灭细菌的能力(P<0.05)。结论 CHX具有明显的杀灭多物种细菌生物膜的作用,但是尚不能达到完美的根管冲洗消毒效果。Objective To evaluate the effect of chlorhexidine digluconate（CHX）,a solution for root canal irrigation,on multispecies biofilms in vitro.Methods CHX（2%）was used to irrigate multispecies biofilms,which were formed on sterile hydroxyapatite disk coated with typeⅠ collagen.Three minutes later,the antibacterial effect was examined by confocal laser scanning microscopy（CLSM）.Results The multispecies biofilms incubated in aerobic and anaerobic hydroxyapatite disks for three weeks grew well.CHX could kill some of the bacterial cells in the multispecies biofilms in both aerobic group（33.0±3.0）%and anaerobic group（40.0±2.8）%,but neither did deionized water（P〈0.05）.Conclusion CHX has bactericidal effects against multispecies biofilms in vitro.However,using CHX alone can not obtain an optimal outcome of root canal irrigation.

关 键 词：多物种生物膜 细菌生物膜 洗必泰葡萄糖酸盐 根管冲洗 牙髓根尖周病 

分 类 号：R781.3[医药卫生—口腔医学]