赤水丹霞来源抗白色念珠菌放线菌分离及进化分析  

Separation and phylogenetic analysis of actinomycetes with activity of against Candida albicans from Chishui danxia soil

作　　者：王苗[1] 李园园[1] 邵美云[1] 罗显涛[2] 吴述铭[2] 曾令荣[2] 胡咏雄 岳昌武[1] 

机构地区：[1]遵义医学院医学与生物学研究中心,贵州省特色微生物资源及药物开发重点实验室,贵州遵义563099 [2]遵义医学院药学院,贵州遵义563099

出　　处：《遵义医学院学报》2014年第4期404-408,共5页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:31160004);贵州省教育厅特色重点实验室建设项目(NO:黔教合KY字[2012]011);贵州省科技学技术基金项目(NO:黔科合J字[2010]2156);(NO:黔科合J字[2012]2348);(NO:黔科合SY字[2013]3013)

摘　　要：目的分离、筛选抗白色念珠菌放线菌菌株,为开发微生物来源的抗白色念珠菌感染药物提供菌株资源。方法采集赤水丹霞山区土壤样品,利用不同的放线菌选择培养基分离、纯化单菌落。提取分离菌株基因组DNA,以细菌16S rRNA通用引物PCR扩增16S rRNA基因,产物测序并比对分析以确定分离菌株的分类地位。小量发酵产物进行抗白色念珠菌活性测试。结果筛选得到7株具有抗白色念珠菌活性的放线菌,其中6株为链霉菌,1株为地中海拟无枝酸菌。结论从赤水丹霞山区分离得到多株具有抗白色念珠菌活性的放线菌,可为开发微生物来源的抗白色链珠菌药物提供菌株来源。Objective To isolate and screen actinomycetes strains with anti - Candida albicans, to provide strain resources for developing anti - Candida albicans drugs from microorganism. Methods Soil of Chi - shui Dan - xia was collected following by separating and pursing single colony using different isolation medium. Genomie DNA was extracted and 16S rRNA gene was amplified by PCR using universal primers 27F/1492R. PCR products were sequenced and compared online in NCBI to determine the taxonomic status. Finally, Anti - Candida activity test of fermentation products were performed. Results 7 Actinomycetes strains, including 6 streptomyces and 1 amycolatopsis, with anti - Candida albieans activity were identified. Conclusion Streptomyces strains derived from Chi - shui Dan - xia have stronger anti - Candida albieans activity, providing material resource for develo- ping anti -Candida albicans agents from microorganism.

关 键 词：白色念珠菌 抗菌活性 16S RRNA 系统发育分析 

分 类 号：R915[医药卫生—微生物与生化药学]