HPLC法测定骨康口服液中阿魏酸的含量  

Determination of Ferulic Acid in Gukangkoufuye by HPLC

作　　者：李赐恩[1] 涂兴明[2] 吴康郁[2] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学附属骨伤科医院,广州510240

出　　处：《北方药学》2014年第10期5-6,共2页Journal of North Pharmacy

基　　金：广东省科技计划项目(2011B031700049)

摘　　要：目的：建立HPLC测定骨康口服液中阿魏酸的含量测定方法。方法：采用HPLC法测定阿魏酸的含量，Diamonsil C18（4.6mm＆#215;250mm，5μm）为色谱柱，流动相为乙腈-0.085%磷酸溶液（17∶83），流速1.0ml·min-1，检测波长316nm。结果：阿魏酸在6.205～24.82μg·ml-1呈良好的线性关系（r=1.0000）。回收率为98.22%（RSD 1.4%，n=9）。结论：本法简便，准确，灵敏度高，重现性好，能有效控制骨康口服液的质量。Objective：To establish a quantitative method for ferulic acid in Gukangkoufuye by HPLC.Method： Using a HPLC method with Diamonsil C18 （4.6mm ＆#215;250mm,5μm）, the mobile phase was acetonitrile-0.085% phosphoric acid solution （17∶83）at a flow rate of 1.0ml＆#183;min-1, the UV detection wavelength was 316 nm.Result： The linear range of ferulic acid was in the range between 6.205μg＆#183;ml-1 and 24.82μg＆#183;ml-1 （r=1.0000）. The average recovery of ferulic acid was 98.22% with RSD of 1.4%.Conclusion： This method is simple, efficient and accurate, which can be used for the quality control of ferulic acid in Gukangkoufuye.

关 键 词：骨康口服液 阿魏酸 高效液相色谱 质量控制 

分 类 号：R927.2[医药卫生—药学]