红镰霉素龙胆二糖苷和异红镰霉素龙胆二糖苷相互转化研究  

Interconversion of Two Naphthopyrone Isomers-Rubrofusarin Gentiobioside and Isorubrofusarin Gentiobioside

作　　者：张杰[1] 张振秋[1] 米宝丽[1] 邓仕任[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国药学杂志》2014年第17期1491-1495,共5页Chinese Pharmaceutical Journal

基　　金：国家中医药行业科研专项"19种生熟异用中药饮片临床规范使用研究(决明子)"(20110700703)

摘　　要：目的研究不同溶剂和温度下决明子中对照品红镰霉素龙胆二糖苷与异红镰霉素龙胆二糖苷相互转化,并对转化产物进行分析鉴定。方法将红镰霉素龙胆二糖苷和异红镰霉素龙胆二糖苷分别置于不同溶剂、不同温度下处理,采用HPLC测定转化产物的含量,计算转化产物的增加率,并利用HPLC-DAD、LC-ESI/MS、NMR对转化产物进行分析。结果在水和氨水溶液中,于一定温度下红镰霉素龙胆二糖苷与异红镰霉素龙胆二糖苷能相互转化;在酸性水溶液中,不能转化。溶剂和温度是影响转化和两者稳定性的重要因素。结论在国内外,首次发现红镰霉素龙胆二糖苷和异红镰霉素龙胆二糖苷能相互转化。OBJECTIVE To study the interconversion of rubrofusarin gentiobioside and isorubrofusarin gentiobioside in Cassiae Semen in different solvents at different temperatures, and identify the structures of the transformation products. METHODS Rubro- fusarin gentiobioside and isorubrofusarin gentiobioside were processed in different solvents at different temperatures respectively. The contents of transformation products were determined by HPLC and the increment rate of were calculated. Then HPLC-DAD, LC-ESI/ MS and NMR were performed to identify the structures of the transformation products. RESULTS Rubrofusarin gentiobioside and isorubrofusarin gentiobioside could convert in water and ammonia at a certain temperature, but the reaction could not happen in acid solvent. On the other hand, solvent and temperature could affect the stability of the two compounds. CONCLUSION This is the first report at home and abroad of the phenomenon that a linear naphthopyrone D-gentiobioside of RG and a non-linear naphthopyrone D-gen- tiobioside of IsoRG could interconvert.

关 键 词：决明子 红镰霉素龙胆二糖苷 异红镰霉素龙胆二糖苷 同分异构体 萘并吡喃酮苷 成分转化 液相色谱-电喷雾串联质谱 核磁共振 

分 类 号：R284[医药卫生—中药学]