7种海洋矿物药的比较分析研究  

Comparative analysis of seven marine biological source of mineral drugs

作　　者：司玮[1,2] 阿如娜[1] 李尚蓉 张静娴[2] 吴婉莹[2] 崔亚君[1] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]中国科学院上海药物研究所,上海中药现代化研究中心,上海201203

出　　处：《中国中药杂志》2014年第17期3321-3325,共5页China Journal of Chinese Materia Medica

基　　金：国家高技术研究发展计划(863)项目(2013AA093002);2015年版药典标准提高项目

摘　　要：《中国药典》2010年版收载的海洋矿物药主要包括珍珠、珍珠母、蛤壳、牡蛎、瓦楞子、海螵蛸、石决明等,该类药材化学成分组成相似,主要是碳酸钙和少量的蛋白质。该文采用EDTA络合滴定法测定碳酸钙的含量;采用X射线粉末衍射,测定其碳酸钙晶型;采用紫外分光光度法对水解后样品中总氨基酸进行测定。通过系统的比较研究,寻找该类药材的差异性,结果显示,所收集的样品中牡蛎所含碳酸钙为三方晶系方解石,石决明及珍珠母晶型为三方晶系方解石与斜方晶系文石混合晶型,其余4种均为斜方晶系文石;所有样品的碳酸钙质量分数在86%~96%;海螵蛸中总氨基酸质量分数最高达1.70%、蛤壳中最低,平均为0.16%。通过对7种海洋矿物药中碳酸钙和总氨基酸的分析,建立了一种对海洋矿物药质量控制的有效方法。The marine biological source of mineral drugs recorded in Chinese Pharmacopoeia （2010 version） mainly including pearl, nacre, clam shell, common oyster shell, ark shell, cuttle bone, and sea-ear shell are widely used in clinical. Calcium carbonate and a small amount of protein are the main components in this type of drugs. In this paper, a systematical and comparable study were carried out by determination of calcium carbonate by EDTA titration method, the crystal of calcium carbonate by X-Ray powder diffraction and the total amino acids （TAAs） of the hydrolyzed samples by ultraviolet spectrophotometry method. As a result, the crystal structure is calcite for common oyster shell, mixture of calcite and aragonite for nacre and sea-ear shell, aragonite for the other drugs. The content of calcium carbonate ranged from 86% to 96%. Cuttle bone has the highest amount of TAAs among the seven drugs which reached 1.7% while clam shell has the lowest content of 0. 16% on average. In conclusion, an effective method was developed for the quality control of marine mineral drugs by comprehensive analysis of calcium carbonate and TAAs in the seven marine mineral drugs.

关 键 词：海洋矿物药 X射线衍射 碳酸钙 氨基酸 EDTA络合滴定 紫外分光光度法 

分 类 号：R282.76[医药卫生—中药学]