改进的线栓法对不同品系小鼠脑缺血模型的构建  

Build of focal cerebral ischemia model in different varieties of mice with modification monofilament

作　　者：贾蔷[1,2] 石作荣[1] 杨洪军[2] 

机构地区：[1]山东中医药大学基础医学院,山东济南250300 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2014年第17期3367-3370,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81330086)

摘　　要：目的:建立不同品系小鼠局灶性脑缺血(MCAO)模型的通用方法。方法:健康雄性昆明(KM)小鼠和C57BL/6小鼠各20只,随机分为对照组10只,MCAO组10只,改良线栓法制作小鼠MCAO模型;大脑中动脉血流监测,栓塞24 h后行神经行为学评分,TTC染色测定脑梗死体积百分比,对模型可靠性进行评价。结果:KM和C57BL/6小鼠MCAO模型组脑血流监测显示梗死处血流基值下降比分别为(81.65±4.59)%,(83.68±6.25)%,神经功能评分分别为(2.30±0.82),(2.50±0.80)分,体征明显,TTC染色可清晰地显示出梗死范围且相对稳定,C57BL/6小鼠有1只TTC染色梗死灶不明显,24 h成活率KM和C57BL/6小鼠分别为100%,80%。结论:颈总插线法制作小鼠脑缺血模型无需开颅,创伤极小,缺血的部位较恒定,从模型制备中线栓、温度、麻醉各个关键环节加以优化和改良,使该模型的制备更加完备。Objective： To establish a general method of focal cerebral ischemia model in different varieties of mice. Method： Each group of healthy adult KM and C57BL/6 mice were randomly divided into control group （n = 10）and MCAO group （n = 10）. The mice in MCAO group were applied in the preparation of the MCAO model by intraluminal occlusion using monofilament. Twenty-four hours after operation, the neurologic function was evaluated, middle cerebral artery blood flow was monitored and the infarction volume was calculated by TIC staining, to evaluate the reliability of the model. Result： In the MCAO group, the base value of the cerebral blood flow down of KM and C57BL/6 mice respectively was（81.65 ±4. 59）%, （83.68 ±6. 25） %. The neurological deficit score respectively was （2. 30 ± 0. 82）, （2. 50±0. 80）. TTC staining can clearly show the infarction area, and relatively stable, 24 hours of the survival rate of KM and C57BL/6 mice were 100% and 80% respectively. Conclusion： The key link is the optimization and improvement of monofilament,temperature, anesthesia and so on. The modified intraluminal occlusion of MCAO using monofilament is a kind of reliable and simple method to establish experimental cerebral ischemia model in mice.

关 键 词：小鼠 大脑中动脉栓塞 动物模型 插线法 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]