天然固定化木瓜脂肪酶催化芳香仲醇动力学拆分  

Kinetic Resolution of Racemic Aromatic Secondary Alcohols Catalyzed by a Naturally Immobilized Carica papaya Lipase

作　　者：苏二正[1] 尤鹏永 魏东芝[2] 

机构地区：[1]南京林业大学轻工科学与工程学院酶与发酵工程实验室,江苏南京210037 [2]华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237

出　　处：《精细化工》2014年第9期1075-1079,1168,共6页Fine Chemicals

基　　金：国家高技术研究发展计划(863计划)项目资助(2012AA022206B);江苏高校优势学科建设工程资助项目(PAPD);南京林业大学引进高层次人才科研基金资助;南京林业大学“青年拔尖人才培养计划”专项经费资助~~

摘　　要：将木瓜来源的廉价天然固定化脂肪酶CPL用于手性拆分芳香仲醇,以4-苯基-2-丁醇作为模式底物,考察了反应温度、反应溶剂、酰基供体以及底物摩尔比对CPL拆分能力的影响,结果表明,适宜的反应温度30～45℃,酰基供体为活性乙烯酯,正己烷或环己烷作为反应溶剂,底物/酰基供体摩尔比1∶2,在该条件下底物的转化率接近50%,e.es大于99%,反应的对映体比值E大于200。CPL对其他结构类似的芳香仲醇也具有很好的拆分能力。该研究为手性芳香仲醇的酶催化制备提供了新的催化剂选择。The low-cost naturally immobilized lipase CPL from the Carica papaya was used to catalyze the kinetic resolution of aromatic secondary alcohols. Taking 4-phenyl-2-butanol as the model substrate, the effects of reaction temperature, reaction solvent, acyl donor and substrate molar ratio on the resolution ability of CPL were investigated. The results show that the optimum reaction conditions were as follows ： reaction temperature of 30 ~ 45 ~C, acyl donor of active vinyl ester, n-hexane or cyclohexane as reaction solvent and substrate/acyl donor molar ratio of 1：2. Under these conditions, the substrate conversion was close to 50%, e. es was more than 99%, and enantiomeric ratio E was higher than 200. CPL also showed good resolution ability for other aromatic secondary alcohols with similar structure. This work provides a new biocatalyst alternative for economic preparation of chiral aromatic secondary alcohols.

关 键 词：木瓜脂肪酶 酶催化 手性 芳香仲醇 动力学拆分 生物工程 

分 类 号：TQ35[化学工程] Q939.9[生物学—微生物学]