离子液体/盐双水相萃取分离锁阳中熊果酸  

Separation of Ursolic Acid from Cynomorium songaricum Rupr by Ionic Liquid /Salt Two Aqueous Phase Extraction

作　　者：张喜峰[1] 罗光宏[2] 张国娜[1] 钟家旺[1] 刘辉[1] 谢斌[1] 

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]河西学院凯源生物技术开发中心甘肃省微藻工程技术研究中心,甘肃张掖734000

出　　处：《精细化工》2014年第9期1080-1084,1090,共6页Fine Chemicals

基　　金：甘肃省中小企业创新基金项目(1110FCCG112);甘肃省微藻工程技术研究中心建设项目(1009FTGG017);Innovation Fund for Small and Medium-sized Technology-based Firms in Gansu Province(1110FCCG112);the Provincial Engineering Technical Research Center for Microalgae in Gansu(1009FTGG017)

摘　　要：利用1-丁基-3-甲基咪唑氯([Bmim]Cl)和磷酸氢二钾(KH2PO4)双水相体系萃取分离锁阳中熊果酸,首先对[Bmim]Cl质量分数、KH2PO4质量分数、熊果酸粗提取液质量分数、NaCl质量分数、pH进行单因素研究,确定双水相体系组成为质量分数25%[Bmim]Cl-21%KH2PO4。然后,利用Box-Benhnken Design实验,对黄酮粗提取液质量分数、pH、NaCl质量分数进行了优化,结果表明:粗提液质量分数8.5%、NaCl质量分数1%、pH=4.7,熊果酸萃取率可达97.85%,与预测值98.3%接近。说明该优化工艺具有可行性。In this study the potential use of ionic liquid-based aqueous two phase system（ILATPS） for the extraction of ursolic acid from Cynomorium songaricum Rupr was evaluated and optimized. The effects of independent variables （the mass fraction of [ Bmim] C1 and KH2PO4, mass fraction of crude extract, mass fraction of NaC1 and pH） were evaluated by single factor analysis. The optimized ATPS of 25% [ Bmim ] Cl-21% KH2PO4 was selected based on their higher upper phase partitioning coefficient and the recovery, Using the optimized ILATPS solvent, the Box-Benhnken Design results show that the mass crude extract 8.5% ,NaC1 1% ,and pH =4. 7 were the optimal ILATPS extraction conditions. The maximum yield of the extracted ursolic acid in the experiments was determined to be 97.85% under the optimal conditions ,which was in good agreement with the predicted one of 98.3%. In conclusion, the optimized process has good reliability.

关 键 词：锁阳 离子液体 双水相 熊果酸 催化与分离提纯技术 

分 类 号：TQ028.32[化学工程]