妇科门诊妇女人乳头瘤病毒感染现状及基因亚型分布  

Human papillomavirus infection situation and its genotype distribution among gynecological outpatients

作　　者：黄江渝[1] 唐建 杨淑哲 刘成桂 

机构地区：[1]成都市第三人民医院检验科,成都610041 [2]成都市妇女儿童中心医院检验科,成都610041

出　　处：《重庆医学》2014年第27期3614-3616,共3页Chongqing medicine

基　　金：四川省卫生厅科研课题基金(120511)

摘　　要：目的：了解妇科门诊妇女宫颈人乳头瘤病毒（HPV）感染现状及基因亚型分布。方法采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术检测HPV感染的基因亚型。结果共检测5052份妇科门诊患者宫颈脱落细胞样本，HPV感染总阳性率为17．52％。各年龄组 H PV感染差异有统计学意义（ P＜0．01），其中20～25岁组的阳性率分别高于26～30岁组和31～35岁组，差异均有统计学意义（ P＜0．01，P＜0．05）；36～40岁组的阳性率高于26～30岁组，差异有统计学意义（ P＜0．05）。高危亚型 HPV基因的阳性检出率（18．1％）高于低危亚型（5．5％），差异有统计学意义（P＜0．01）。高危亚型中以HPV52亚型最多，占15．03％，其次是HPV16和 HPV58亚型；低危亚型以 HPV81亚型居多，占7．98％。结论妇科门诊妇女HPV感染阳性率较高，且以高危亚型居多；建议针对不同年龄群开展HPV基因亚型的常规检测。＇ Objective To understand the status quo of human papillomavirus （HPV） infection and its genotypes distribution a-mong gynecological outpatients in Chengdu region .Methods The DNA microarray technique combined with PCR and DNA reverse hybridizatio technology was used to detect the genotypes of HPV infection .The data were analyzed by the SPSS 13 .0 software .Re-sults A total of 5 052 samples of cervical exfoliated cells among gynecological outpatients were detected ,and the total positive rate of HPV infection was 17 .52% .The differences of HPV infection among various age groups were statistically significant ,especially the positive rate of HPV infection in the 20-25 years age group was higher than that in the 26-30 years age group and the 31-35 years age group（P〈0 .05） ,and which in the 36 -40 years age group was also higher than that in the 26 -30 years age group （P〈0 .05） .The positive detection rate of high risk HPV subgenotypes was 18 .1% ,which was higher than 5 .5% of low risk sub-genotypes with statistical difference （P〈0 .01） .HPV 52 was the most frequent subgenotypes in high risk subgenotypes ,accounting for 15 .03% ,followed by HPV 16 and HPV 58;HPV 81 was the most frequent subgenotypes in low risk subgenotypes ,accounting for 7 .98% .Conclusion The positive rate of HPV infection among gynecological outpatients is higher ,and the majority of geno-types are high risk .It is suggested that the routine examination of HPV subgenotypes detection focused on different age groups should be recommended .

关 键 词：人乳头瘤病毒亚型检测 PCR-反向点杂交 DNA芯片技术 妇科门诊 

分 类 号：R737.33[医药卫生—肿瘤]