建兰花叶病毒贵州分离物的分子鉴定  

Molecular identification of Cymbidium mosaic virus isolated from Paphiopedilum dianthum in Guizhou (China)

作　　者：李欲轲 洪鲲[1] 张宇斌[1] 乙引[1] 万晴姣[1] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《植物检疫》2014年第5期67-70,共4页Plant Quarantine

基　　金：长江学者和创新团队发展计划资助(PCSIRT);贵州省重点实验室项目[黔科合计Z字[2011]4005号];贵阳市星火计划项目[(2010)筑科农合同字第1-农-23号];贵州省社发攻关项目[黔科合SZ字(2011)3100号];贵州省农业攻关项目[黔科合NY字(2010)3031];贵州省科技联合基金[黔科合J字LKS(2010)17]

摘　　要：采用生物学方法对ELISA检测为阳性的建兰花叶病毒贵州长瓣兜兰分离物(CyMV-GZ)进行纯化。RT-PCR技术克隆其外壳蛋白(CP)基因并测序,使用DNAMAN7.0和MEGA5.0软件对CyMV-GZ的CP基因进行同源性分析。结果表明:成功克隆该病毒CP基因,其序列长度为672 bp,编码223个氨基酸残基;序列同源性分析显示,该分离物的CP基因与16种已报道的CyMV各地分离物同源性均达95%以上,而与同属其他3种病毒CP基因同源性均低于48%,由此证明该长瓣兜兰分离物为CyMV。A suspected Cymbidium Mosaic Virus （CyMV） was isolated from Paphiopedilum dianthum cultivated in Guizhou Academy of Forestry with mosaic symptom by biological method. The coat protein （CP） gene of the virus isolate was then cloned, sequenced and analyzed. The results showed that the CP gene, with length of 672 bp, encodes a 223-amino acid CP protein. Sequence analysis based on the CP gene showed that the nucleotide sequence of the isolate shared over 95% homology with 16 reported CyMV isolates, while less than 50% with other three viruses belonging to Potexvirus, demonstrating that the virus isolated from Paphiopedilum dianthum is CyMV.

关 键 词：建兰花叶病毒 外壳蛋白 分子鉴定 长瓣兜兰 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]