邻苯二甲酸二(2-乙基己)酯对小鼠肺细胞DNA的损伤作用  

DNA damage induced by di(2-ethylhexyl) phthalate in mice lung cells

作　　者：王海雪[1] 冯斐斐[1] 高留闯[1] 巴月[1] 范清堂[1] 段丽菊[1] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450001

出　　处：《癌变．畸变．突变》2014年第5期386-388,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨邻苯二甲酸二(2-乙基己)酯(DEHP)对小鼠肺细胞DNA的损伤作用。方法:以生理盐水作为阴性对照组,用不同浓度DEHP(5、25、125、625μmol/L)对小鼠肺细胞进行体外染毒,应用单细胞凝胶电泳技术检测肺细胞DNA的断裂程度。结果:当DEHP的浓度为125和625μmol/L时,肺细胞尾部DNA百分含量、尾矩和Olive尾矩与阴性对照组相比均升高,差异具有统计学意义(P<0.05)。结论:DEHP在体外可造成小鼠肺细胞的DNA断裂损伤。OBJECTIVE：To studyDNA damage induced by di （2-ethylhexyl） phthalate （DEHP） to mice lung cells.METHODS：The negative control group was exposed to saline. Extracted mice lung cells were exposed to different concentrations of DEHP（5,25,125,625μmol/L） and single cell gel electrophoresis was used to test the DNA breakage degree.RESULTS：Compared with the control group,tail DNA percentage,tail moment and Olive tail moment of 125 μmol/L and 625μmol/L DEHP-treated groups were increased significantly （P〈0.05）.CONCLUSION： DEHP could induce DNA breakage of mice lung cells in vitro.

关 键 词：邻苯二甲酸二(2-乙基己)酯 单细胞凝胶电泳 肺细胞 DNA损伤 

分 类 号：R394.2[医药卫生—医学遗传学]