Aβ在两种阿尔茨海默病转基因小鼠模型脑杏仁核分布的比较研究  

Comparative Study on the Distribution of Aβ in Amygdale of Two Transgenic Mice Models of Alzheimer's Disease

作　　者：周奕 邓志伟 艾卫敏[2] 朱耀峰[3] 张剑峰[4] 卢璨[4] 雷德亮[5] 

机构地区：[1]长沙卫生职业学院基础学科部,中国长沙410100 [2]湘潭职业技术学院护理学院,中国湘潭411012 [3]吉首大学医学院,中国吉首416000 [4]中南大学湘雅医学院,中国长沙410013 [5]中南大学基础医学院,中国长沙410013

出　　处：《湖南师范大学自然科学学报》2014年第5期26-30,共5页Journal of Natural Science of Hunan Normal University

基　　金：湖南省卫生厅科研基金课题资助项目(B2014-151);湖南省教育厅科学研究资助项目(14C0091);米塔尔学生创新资助项目(10MX19);长沙市2013年度指导性科技计划资助项目(K13ZD046-33)

摘　　要：目的:比较研究Aβ在两种AD转基因小鼠模型脑杏仁核分布的差异.方法:采用18月龄雄性APP/PSl双转基因(2×Tg-AD)小鼠与同龄同性别APP/PSl/tau三转基因(3×Tg-AD)小鼠,分别进行6E10单克隆抗体免疫组化染色等方法显示Aβ阳性神经元及斑块,观察其分布与形态等的差异,图像分析系统定量比较其量的变化.结果:在杏仁核2×Tg-AD组Aβ阳性产物主要位于细胞外成为细胞外Aβ(eAβ),形成大量的Aβ阳性斑,Aβ阳性神经元少;而3×Tg-AD组Aβ阳性产物主要位于神经元细胞内,成为细胞内Aβ(iAβ),但Aβ阳性斑少见.结论:2×Tg-AD组与3×Tg-AD组Aβ阳性产物在杏仁核分布的差异可能反映了两种AD小鼠模型神经病理等改变的不同.To compare the distribution and morphological features of Aβ inmmnoreactive products in the amygdale of two transgenic mice models of Alzheimer＇s disease. Methods： 18-month-old APP/PS1 （2× Tg- AD） and the age-matched male mice of APP/PSUtau（3 × Tg-AD） were used in this experiment. 6El0 immnohis-tochemical staining was used to show the distribution and morphological features of Aβ immuno×active products, and quantitative analysis we× made on the difference of the products by an image analysis system. Results：The deposition of Aβ in the amygdala of 2 × Tg-AD was mainly seen as extracellular Aβ （eAβ）, and formed a lot of positive plaques; the positive products we× distributed as intracellularly Aβ（iAβ） for the 3×Tg-AD, but the Aβ positive plaques were fairly seen. Conclusion ：The difference of Aβ positive products distribution between the 2 ×Tg- AD and 3 × Tg-AD is likely to be related to the difference of neuropathological changes of these two AD models.

关 键 词：阿尔茨海默病 转基因小鼠 Β淀粉样蛋白 杏仁核 

分 类 号：R749.16[医药卫生—神经病学与精神病学]