放线菌R2A14A-1的活性代谢产物分离及菌株鉴定  

Identification of strain R2A14A-1 and its bioactive secondary metabolite

作　　者：王飞飞[1] 庹利 刘佳萌[1] 刘少伟[1] 董艳萍[2] 蒋忠科[1] 夏曼[2] 李小俊[1,3] 陈川[1] 戴素娟[1] 许敏[1,4] 姜蓉[1] 孙承航[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050 [2]中国药科大学生命科学与技术学院,南京210009 [3]河北北方学院医学检验学院,张家口075000 [4]桂林医学院,桂林541004

出　　处：《中国抗生素杂志》2014年第10期721-728,共8页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(81172963和81373308);北京市自然科学基金(7133249);十二五"重大新药创制"科技重大专项(2012ZX09301-002-001-018);2013年度中央级公益性科研院所基本科研业务专项(IMBF201204;IMBF 201304);高等学校博士学科点专项科研基金博导类项目(NO.20111106110032)和高等学校博士学科点专项科研基金新教师类项目(NO.20131106120027)

摘　　要：目的抗金黄色葡萄球菌化合物FFF-13的分离鉴定及其塔克拉玛干沙漠南麓来源产生菌R2A14A-1的初步鉴别。方法以琼脂平板扩散法和化合物紫外特征追踪R2A14A-1发酵液中抗金黄色葡萄球菌的amicoumacin类化合物,通过大孔吸附树脂和高效液相等色谱法建立空白培养基与菌株发酵液比对指纹图谱,通过比对分离获得活性次级代谢产物FFF-13,根据高分辨质谱、核磁共振氢谱和碳谱数据并结合文献确定化合物FFF-13的结构;综合菌株R2A14A-1的形态特征、培养特征、生理生化特性和16S rRNA基因序列分析对菌株R2A14A-1进行初步鉴定。结果化合物FFF-13为amicoumacin B,菌株R2A14A-1为拟诺卡菌属放线菌。结论菌株R2A14A-1为首个发现产生amicoumacin类抗生素的拟诺卡菌株。Objective Isolation and structural identification of secondary metabolite FFF-13 with anti- Staphylococcus aureus from the culture broth of strain R2A14A-1 isolated from the south edge of the Taklamakan Desert in Xinjiang province in China, and preliminary identification of its producing strain R2A14A-1. Methods Based on paper disc diffusion assay and UV spectra, HPLC fingerprint was established to identify the secondary metabolites by comparison of the fermentation broth with the blank culture medium after macroporous resin absorption and acetone water solution elution. Compound FFF-13 was identified as an active secondary metabolite and finally purified by HPLC. The chemical structure of FFF-13 was determined by HR-ESI-MS, NMR analysis and data comparisons with that of the related literature. Preliminary identification of strain R2A14A-1 was carried out by the method of comprehensive analysis of its phylogenetic relationships, combined with morphological, cultural and physiological characteristics. Results Compound FFF-13 was identified as amicoumacin B and the strain R2A14A-1 was affiliated with the genus Nocardiopsis. Conclusion To our best knowledge, it is the firstly reported that a Nocardiopsis strain can produce amicoumacin group antibiotics.

关 键 词：拟诺卡菌 Amicoumacin B 16S RRNA 鉴别 

分 类 号：R978[医药卫生—药品]