利用MEP途径过表达提高β-胡萝卜素产量  

Improvement of β-Carotene Production by Overexpression MEP Pathway

作　　者：陈习平 谢丽萍[1] 张琪[1] 胡又佳[1] 

机构地区：[1]中国医药工业研究院总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2014年第10期929-933,共5页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2011ZX09203-001-06);上海自然科学基金(11ZR1435200);上海市科委科技支撑项目(13431900204)

摘　　要：构建上游甲基赤藓糖-4-磷酸(MEP)途径,获得两种重组质粒pET32a(+)-dxs-idi-ispDF和pTrcHis2B-dxs-idiispDF,然后与下游β-胡萝卜素合成途径的质粒pACYC184-M-crt一起转化进入大肠杆菌中,获得相应的重组菌株。结果表明含有MEP途径的重组菌株β-胡萝卜素产量有显著提高。An upstream pathway named methyl-erythrose 4-phosphate （MEP） pathway was constructed, and two kinds of recombinant plasmid pET32a （＋） -dxs-idi-ispDF and pTrcHis2B-dxs-idi-ispDF were obtained. Then the upstream pathway and a downstream β-carotene-forming pathway were transformed into Eseherichia coli to get two kinds of recombinant strains. The results suggested that the β-carotene production of the recombinant strains contained MEP pathway were significantly improved.

关 键 词：甲基赤藓糖-4-磷酸(MEP)途径 Β-胡萝卜素 过表达 

分 类 号：Q78[生物学—分子生物学]