miR-155特异性siRNA增强阿糖胞苷诱导的Burkitt淋巴瘤Raji细胞凋亡  

miR-155-specific siRNA enhances chemosensitivity of Burkitt lymphoma Raji cells to cytosine arabinoside by inducing apoptosis

作　　者：刘平平 朱锦灿[2] 郑力 刘善淘 谭广销[2] 何冬梅[2] 刘革修[2] 

机构地区：[1]清华大学第一附属医院血液肿瘤科,北京100016 [2]暨南大学医学院血液病研究所,广东广州510632

出　　处：《中国病理生理杂志》2014年第8期1374-1378,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81270568)

摘　　要：目的:研究miR-155特异性siRNA增强阿糖胞苷(Ara-C)诱导的Burkitt淋巴瘤Raji细胞凋亡及其机制。方法:Ara-C与miR-155 siRNA单独或者联合干预Raji细胞增殖。qRT-PCR检测脂质体转染siRNA后miR-155的表达;CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting法检测caspase-3的表达。结果:qRT-PCR显示,转染miR-155 siRNA后,Raji细胞的miR-155表达明显下降(P<0.05),Ara-C或miR-155 siRNA单独处理细胞后均显示出增殖抑制作用,而两者联合使用的增殖抑制作用更显著(P<0.05)。各组细胞进行药物干预48 h后行流式细胞术,结果显示Ara-C或miR-155 siRNA单独处理细胞凋亡率分别为(16.5±0.3)%和(14.6±0.3)%,与对照组[(3.6±0.4)%]比较差别有统计学意义(P<0.05),Ara-C+miR-155 siRNA组的凋亡率[(38.4±1.4)%]分别与Ara-C和miR-155 siRNA单独处理组比较,差别有统计学意义(P<0.05)。Ara-C+miR-155siRNA组与Ara-C组及miR-155 siRNA组相比,caspase-3的水平明显增高。结论:miR-155特异性siRNA具有增强阿糖胞苷对Raji细胞增殖抑制及诱导Raji细胞凋亡的作用,其机制与caspase-3凋亡途径有关。[ABSTRACT]AIM：ToinvestigatetheeffectofmiR-155-specificsiRNAaloneorincombinationwithcytosinear-abinoside （Ara-C） on the growth and apoptosis of Burkitt lymphoma Raji cells .METHODS： miR-155-specific siRNA and/or Ara-C were used to treat the cells .Quantitative real-time polymerase chain reaction was used to detect the expres-sion of miR-155.The growth of the cells was analyzed by CKK-8 assay.The cell apoptosis was determined by flow cytome-try.RESULTS：The miR-155 expression level of the cells transfected with miR-155 siRNA was significantly lower than that in the 2 control groups .Ara-C or miR-155 siRNA alone inhibited the growth of Raji cells in a dose-depend manner . miR-155 siRNA combined with Ara-C produced more inhibition of cell proliferation （P＆lt;0.05）.After treatment for 48 h, the apoptotic rate of Raji cells in miR-155 siRNA＋Ara-C group [（38.4 ±1.4）%] was higher than that in Ara-C group [（16.5 ±0.3）%] and miR-155 siRNA group [（14.6 ±0.3）%], with statistically significant difference （P＆lt;0.05）. The expression of caspase-3 in Ara-C＋miR-155 siRNA group was increased significantly as compared with Ara-C group and miR-155 siRNA group.CONCLUSION：miR-155-specific siRNA enhances the chemosensitivity of Raji cells to Ara-C by inducing apoptosis through the caspase-3 pathway .

关 键 词：阿糖胞苷 MIR-155 BURKITT淋巴瘤 细胞凋亡 

分 类 号：R733.71[医药卫生—肿瘤]