益生乳酸菌对奶牛阴道上皮细胞粘附作用的研究  

Adherence properties of Lactobacillus to the vaginal epithelial cell of cows

作　　者：赵静[1] 李向楠[1] 王军[1] 孙城涛[1] 杨雨江[1] 王全凯[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国预防兽医学报》2014年第9期701-703,739,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：吉林省科技厅项目(20125037);吉林省教育厅项目[吉教科合字(2013)第50号];吉林省现代农业产业技术体系(201327);吉林省科技厅重大科技攻关专项

摘　　要：益生菌对奶牛阴道上皮细胞粘附能力的强弱是其发挥益生作用的前提条件,因此粘附能力是益生菌筛选的重要标准之一。为筛选出可以制备微生态制剂的益生菌菌株,本实验首先通过碳氢化合物粘着法测试了魏斯氏菌(1#菌)、海氏肠球菌(3#菌)、乳酸明串珠菌(5#菌)和营养泥杆菌(9#菌)的疏水性能,随后将细菌作用于奶牛阴道上皮细胞并且利用革兰氏染色法对细菌的粘附能力进行研究。结果表明,疏水率由强到弱依次为:9#菌、5#菌、3#菌和1#菌,其中9#菌对3种溶剂的疏水率均显著高于其它3株细菌(p<0.05);单一菌株中,9#菌株的粘附性显著高于1#、3#和5#菌株(p<0.05),混合菌株1+3#的粘附能力显著高于其它混合菌株和单一菌株(p<0.05)。研究结果提示,1+3#混合菌株更适合用于微生态制剂的研发。Adhesion ability of probiotic bacteria to epithelial cells from cow reproductive tract is the precondition to play a probiotic role, therefore, adhesion ability is one of the important standards of probiotics screening. Firstly, hydrophobicity of the Weissella koreensis （1#）, Enterococcus hirae （3#）, Leuconostoc Lactis （5#） and Ilyobacter polytropus （9#） were tested using the hydrocarbon gelling method （MATH）. Secondly, bacteria were mixed with cow vaginal epithelial cells to investigate the bacterial adhesion ability using gram staining method. The results showed that hydrophobic effect of 9# strain was significantly higher than that of 5#, 3# and 1# strain （p〈0.05）. The adhesion ability of 9# strain was the highest among the four single strains and that of 1＋3# mixed strains was the highest among the single strains and mixed strains （p〈0.05）. These results indicate that 1＋3# mixed strains have the potential to be used as proriotics exploitation.

关 键 词：乳酸菌 阴道上皮细胞 疏水性 粘附 

分 类 号：S852.61[农业科学—基础兽医学]