miR-21对乳腺癌细胞MCF-7增殖的影响  

Impact of miR-21 on proliferation of breast cancer cell MCF-7

作　　者：刘文新[1] 李月峰[1] 朱小兰[2] 王冬青[1] 殷瑞根[1] 赵亮[1] 石俊[1] 赵天[1] 

机构地区：[1]江苏大学附属医院影像科,212001 [2]江苏大学第四附属医院妇产科

出　　处：《江苏医药》2014年第17期1998-2001,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK20131241);江苏省妇幼科研项目(F201215);镇江市科技支撑项目(SH2012057);江苏大学临床科技发展基金(JLY2010153)

摘　　要：目的探讨微小RNA-21(miR-21)对乳腺癌细胞MCF-7增殖的影响。方法实时荧光定量PCR(qRT-PCR)检测15例乳腺癌患者癌组织和癌旁组织中miR-21的表达。乳腺癌细胞株MCF-7用miR-21抑制物转染(抑制组,MCF-7+INH)、抑制物对照转染(对照组,MCF-7+INH-NC)和未转染(空白对照组)后,MTT法检测细胞增殖,流式细胞术分析细胞周期,Western blot检测程序性细胞凋亡因子4(PDCD4)表达。结果 miR-21在乳腺癌组织中高表达(P<0.05)。与对照组相比,miR-21抑制组MCF-7细胞的增殖抑制,G0/G1期细胞比例增多,S期细胞比例减少,PDCD4表达增加(P<0.05)。结论抑制乳腺癌细胞MCF-7中miR-21的表达,可以抑制肿瘤细胞的增殖。Objective To explore the impact of microRNA-21（miR-21）on the proliferation of breast cancer cell MCF-7.Methods The expression of miR-21 in carcinoma tissues（group A）and tumor-adjacent tissues（group B）of 15 patients with breast cancer was detected by qRT-PCR.After MCF-7cell lines were transfected with miR-21inhibitor（group C）and not transfected（group D）,the cell proliferation and cell cycle of MCF-7were detected by MTT and flow cytometry,respectively.The expression of programmed cell death factor 4（PDCD4）in MCF-7 was detected by Western blot.Results The expression of miR-21 was higher in group A than that in group B（P0.05）.Compared with group D,cell proliferation of MCF-7 was inhibited,cell proportion in G0/G1 phase was increased,cell proportion in S phase was decreased,and the expression of PDCD4 was higher in group C（P0.05）.Conclusion Inhibiting the expression of miR-21 in breast cancer cell MCF-7can suppress tumor cell proliferation.

关 键 词：乳腺癌 微小核糖核酸-21 MCF-7细胞 

分 类 号：R737[医药卫生—肿瘤]