L-亮氨酸产生菌的代谢工程改造及其发酵效率  

Metabolic Engineering Modifications of the L-leucine Producing Strain Corynebacterium glutamicum and Its Fermentation Efficiency

作　　者：黄钦耿[1,2] 

机构地区：[1]福建省麦丹生物集团有限公司福州研究中心,福建福州350008 [2]福建师范大学工业微生物发酵技术国家地方联合工程研究中心,福建福州350107

出　　处：《台湾农业探索》2014年第4期68-72,共5页Taiwan Agricultural Research

摘　　要：以选育的获得产L-亮氨酸的谷氨酸棒杆菌MD106为出发菌株,通过重叠延伸PCR及自杀载体介导的同源重组技术构建panBC及alaT双基因缺失的突变株MD106ΔpanBC/ΔalaT,并对出发菌及双缺失重组菌进行摇瓶发酵试验,测定发酵指数。结果显示,发酵40h后,突变株的L-亮氨酸的产量为7.91g/L,比出发菌株提高43.3%,而且主要杂酸——丙氨酸减少超过80%,总杂酸比例较出发菌株减少55.6%。Taking Corynebacterium glutamicurn MD106 as a starting strain applied to produce L-leucine, the mutant strain MD106△pan13C/AalaT from the double gene-negative of panBC and alaT was structured by using the methods of overlap extension PCR and suicide vector-mediated homologous recombination, and the fermentation index was measured through the ermentation test of the starting strain and the recombinant strain. The results showed that the L-leueine yield of the recombinant strain was 7.91g/L by Amino acid analyzer after40 h fermentation, and it increasing by 43.3 % comparing with that of the original strain MD106, and mainly miscellaneous acid--alanine reduced by more than 80%, the total ration of miscellaneous acid decreased 55.6% compared with the original strain.

关 键 词：L-亮氨酸 谷氨酸棒杆菌 代谢工程 杂酸 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]