几种植物精油对红阳猕猴桃中两种致病菌抑制效果的研究  

Inhibitory Effects of Several Plant Essential Oils Against Two Pathogens of 'Red Sun' Kiwifruit

作　　者：李素清[1] 何靖柳[1] 秦文[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《四川农业大学学报》2014年第3期315-320,共6页Journal of Sichuan Agricultural University

基　　金：四川省质检局项目(ZYBZ2013-41);芦山县县校合作项目

摘　　要：【目的】通过研究植物精油在红阳猕猴桃致病菌上的抑制效果,筛选出对红阳猕猴桃有良好贮藏保鲜效果的植物精油。【方法】采用培养基稀释法,利用体积浓度为10.0×104μL/L(v/v)不同植物精油培养基筛选出对红阳猕猴桃中两种致病菌具有抑制效果的精油,并研究其最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。【结果】肉桂精油、百里香精油、丁香精油对尖孢炭疽菌(Colletorichum acutatum)最低抑菌浓度分别为0.125×104,0.500×104,1.00×104μL/L,最低杀菌浓度分别为0.125×104,1.25×104,1.00×104μL/L;肉桂精油、薄荷精油对葡萄座腔菌(Botryosphaeria parva)最低抑菌浓度分别为0.125×104,2.50×104μL/L,最低杀菌浓度分别为0.125×104μL/L和>10.0×104μL/L。【结论】肉桂精油、百里香精油、丁香精油对尖孢炭疽菌(Colletorichum acutatum)有较好抑制作用;肉桂精油对葡萄座腔菌(Botryosphaeria parva)具有良好的抑菌及杀菌作用,而薄荷精油对该菌有一定的抑制作用,但杀菌效果不明显。[Objective] The aim of the study was to investigate inhibitory effects of plant essential oil on pathogens in the ＂Red Sun＂ kiwifruit. In addition, the plant essential oil that had good freshkeeping effect was selected. [Method] In this study, the dilution method was used to select the essential oils which had inhibitory effects against two pathogens of ＂Red Sun＂ kiwifruit. Moreover, the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of essential oils were determined. [Results] The minimal inhibitory concentration of cinnamon essential oil, thyme oil and clove oil against Colletotrichum acutatum were 0. 125 ）K 10^4 , 0. 500 × 10^4 and 1.00X10^4 I^L/L, respectively. The minimum bactericidal concentrations were 0. 125 X 10^4, 1. 25 ）〈 10^4 and 1.00X 10^4 I^L/L, respectively. The minimal inhibitory concentrations of cinnamon and peppermint essential oils against Botryosphaeria parva were 0. 125 × 10^4 and 2.50 × 10^4 /~L/L. The minimum bactericidal concentrations were 0. 125 ）× 10^4 μL/L and 10.0 × 10^4 μL/L, respectively. [Conclusion] Cinnamon essential oil, thyme oil, clove oil had good inhibitory effect against Colletotrichum acutatum. Cinnamon essential oil had high inhibitory activity against Botry- osphaeria parva but peppermint essential oil was not obvious.

关 键 词：植物精油 抑制效果 最低抑菌浓度 最低杀菌浓度 

分 类 号：S436.634[农业科学—农业昆虫与害虫防治]