Tetramethylpyrazine protects lymphocytes from radiationinduced apoptosis through nuclear factor-κB  

作　　者：WANG Xiao-Yan MA Zeng-Chun WANG Yu-Guang TAN Hong-Ling XIAO Cheng-Rong LIANG Qian-De TANG Xiang-Lin CHENG Yu GAO Yue 

机构地区：[1]Institute of Radiation Medicine,Academy of Military Medical Science [2]Chongqing Maternal and Child Health-Care Hospital

出　　处：《Chinese Journal of Natural Medicines》2014年第10期730-737,共8页中国天然药物（英文版）

基　　金：supported by the National Nature Sciences Foundation of China(Nos.81073028,81130067 and 81274127)

摘　　要：AIM: Radiation induces an important apoptosis response in irradiated organs. The objective of this study was to investigate the radioprotective effect of tetramethylpyrazine(TMP) on irradiated lymphocytes and discover the possible mechanism of protection. METHOD: Lymphocytes were pretreated for 12 h with TMP(25-200 μmol·L-1) and then exposed to 4 Gy radiation. Cell apoptosis and the signaling pathway were analyzed. RESULTS: Irradiation increased cell death, DNA fragmentation, activated caspase activation and cytochrome c translocation, downregulated B-cell lymphoma 2(Bcl-2) and up-regulated Bcl-2-associated X protein(Bax). Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit caspase-9 activation, and upregulate Bcl-2 and down-regulate Bax in 4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB(NF-κB) from cytosol into the nucleus. CONCLUSION: The results suggest that TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis.AIM： Radiation induces an important apoptosis response in irradiated organs. The objective of this study was to investigate the radioprotective effect of tetramethylpyrazine（TMP） on irradiated lymphocytes and discover the possible mechanism of protection. METHOD： Lymphocytes were pretreated for 12 h with TMP（25-200 μmol·L-1） and then exposed to 4 Gy radiation. Cell apoptosis and the signaling pathway were analyzed. RESULTS： Irradiation increased cell death, DNA fragmentation, activated caspase activation and cytochrome c translocation, downregulated B-cell lymphoma 2（Bcl-2） and up-regulated Bcl-2-associated X protein（Bax）. Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit caspase-9 activation, and upregulate Bcl-2 and down-regulate Bax in 4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB（NF-κB） from cytosol into the nucleus. CONCLUSION： The results suggest that TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis.

关 键 词：TETRAMETHYLPYRAZINE RADIOPROTECTION NF-κB APOPTOSIS 

分 类 号：R965[医药卫生—药理学]