磺胺二甲嘧啶间接竞争化学发光酶免疫分析法的建立及应用  

Development and application of indirect competitive chemiluminescence immunoassay for detecting sulfadimidine residue

作　　者：马玲[1] 韦建兴[1] 陆忠础 唐承明 李辉 李智红 张怡轩[1] 甘浪帆 吴健敏[1] 

机构地区：[1]广西壮族自治区兽医研究所,广西南宁530001 [2]南宁市动物卫生监督所,广西南宁530001 [3]广西壮族自治区兽药监察所,广西南宁530001

出　　处：《中国食品卫生杂志》2014年第5期455-460,共6页Chinese Journal of Food Hygiene

基　　金：广西壮族自治区科技厅基本科研业务专项(桂科专项12-4);南宁市科学研究与技术开发计划-科技创新能力与条件建设项目(201102047B)

摘　　要：目的建立一种更加灵敏的磺胺二甲嘧啶（SM2）免疫学检测方法。方法本研究制备抗SM2单克隆抗体（SM2-mAb）,用于建立SM2间接竞争化学发光酶免疫分析法（SM2-CLEIA）,并检测主要动物食品中SM2残留。结果本研究制备的SM2-mAb为IgG2b亚类,亲和常数为0.12×107L/mol;SM2-CLEIA曲线在0.1～1 000μg/L之间呈现良好的线性关系,相关系数r2=0.990 4,半数抑制浓度（IC50）为4.006μg/L,检测限为0.174μg/L,添加回收率为94.41%～104.40%,批内和批间变异系数分别为3.07%和8.22%,与磺胺脒等药物无交叉反应,与SM2-ELISA试剂盒的阴阳性符合率为100%。结论建立了灵敏、特异、准确、检测范围宽的SM2-CLEIA检测方法。Objective To develop a more sensitive immunologic method for SM2 detection. Methods An indirect competitive chemiluminescence immunoassay was established with SM2 monoclonal antibody, and was used to detect the SM2 residue in animal derived foods. Results The results indicated that the SM2-mAb belongs to subclass of IgG2b, and its affinity constant was 0. 12-- 107 L/mol. The detection limit of SM2-CLEIA was 0. 174μg/L with the linear range of 0. 1 to 1 000 μg/L （r2 =0. 990 4） , and IC5o was 4. 006 μg/L. The recoveries were from 94.41% to 104.40%. The variability within and between batches were 3.07% and 8.22% , respectively. There was no significant cross reaction between SM2-mAb and SG or other drugs, and the results were 100~ in accordance with those of the SME-ELISA kit. Conclusion SME-CLEIA was established, which was sensitive, specific, precise and with wide linear range.

关 键 词：磺胺二甲嘧啶 间接竞争化学发光酶免疫分析法 兽药残留 检测 

分 类 号：S859.84[农业科学—临床兽医学]