Isolation and Characterization of Fucoidans from Five Brown Algae and Evaluation of Their Antioxidant Activity  

作　　者：QU Guiyan LIU Xu WANG Dongfeng YUAN Yi HAN Lijun 

机构地区：[1]Department of Food Science and Engineering, Ocean University of China [2]Institute of Oceanology, Chinese Academy of Sciences [3]College of Tea & Food Science and Technology, Anhui Agricultural University

出　　处：《Journal of Ocean University of China》2014年第5期851-856,共6页中国海洋大学学报（英文版）

基　　金：supported by the Ocean Public Welfare Scientific Research Project, State Ocean Administration of China (No. 201105029-9)

摘　　要：In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica（LJF）, Lessonia nigrescens（LNF）, Lessonia trabeculata（LTF）, Ascophyllum mackaii（AMF）, and Ecklonia maxima（EMF）. LJF was less in sulfate content（14.16%） and more in galactose and mannose content（1.08 and 0.68） than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans（1.21–1.41）. AMF（162 kDa） and EMF（150 kDa） were the first two largest in molecular weight, which were followed by LJP（126 kDa）, LNF（113 kDa） and LTF（105 kDa）. The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.

关 键 词：fucoidans composition antioxidant property Laminaria japonica Lessonia nigrescens Lessonia trabeculata Ascophyllum mackaii Ecklonia maxima 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]