外源性H_2S供体NaHS对心肺复苏后大鼠学习记忆能力的影响  

Effects of exogenous H_2S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation

作　　者：吴长江[1] 黄超发 朱团结[1] 汪惠[2] 

机构地区：[1]上海交通大学医学院苏州九龙医院重症医学科 [2]第二军医大学附属长海医院麻醉科,上海200433

出　　处：《中国临床药理学与治疗学》2014年第7期727-732,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究外源性H2S对心肺复苏(cardiopulmonary resuscitation,CPR)后大鼠记忆障碍的影响,并探究其作用机制。方法:将SD大鼠随机分为5组,对照组、模型组(CPR)、低剂量NaHS治疗组(CPR+NaHS)、中间剂量NaHS治疗组、高剂量NaHS治疗组。采用Morris水迷宫法测定各组大鼠学习记忆能力;比色法检测大鼠大脑组织中超氧化物歧化酶(superoxide dismutase,SOD)活性及丙二醛(malonaldehyde,MDA)含量;HE染色观察海马组织形态学改变。结果:与对照组比较,模型组大鼠在Morris水迷宫实验中,逃避潜伏期显著延长,在平台所在象限停留时间减少,穿越平台次数减少,SOD活性显著降低,MDA含量显著升高,海马神经元出现变性改变。与模型组比较,NaHS治疗组大鼠逃避潜伏期缩短,在平台所在象限搜索时间延长,穿越平台次数明显增多,SOD活性升高,MDA含量显著降低,海马神经元的变性得到改善。结论:心肺复苏可损害大鼠的学习记忆能力,外源性H2S供体NaHS减轻了CRP对大鼠学习记忆的损害,其作用机制可能与H2S清除自由基和衰减脂质过氧化反应有关。ABSTRACT AIM. To investigate the effects of exogenous hydrogen sulfide on learning and memory in rats after cardiopulmonary resuscita- tion（CPR） and explore its mechanism. METH- ODS： Experimental rats were randomly divided into five groups： control group, model group （CPR）, NariS low dose group, middle dose group and high dose group . The ability of learning and memory was tested by Morris water maze; The activity of super oxide dismutase （SOD） and the content of malondialdehyde （MDA） were measured with colorimetry; the morphological alterations in hippocampus slices were assessed by microscope. RESULTS： Com- pared with control group, the escape latency in model group was longer, the residence time in o- riginal platform quadrant and the times of pass- ing the position of platform were less, SOD ac- tivity was decreased and the content of MDA was increased in model group. Compared withmodel group, the escape latency in treatment groups was shorter, the number of crossings o- ver the target area and the residence time in o- riginal platform quadrant were increased. SOD activity and the content of MDA was increased and decreased, respectively. Morphological changes were significantly attenuated by Naris in treatment groups. CONCLUSION. CPR can impair the ability of learning and memory of rats, and exogenous H2S donor Naris can re duce the impairment of learning and memory of rats after CPR. The mechanism may be related to that H2 S has the effects of anti-lipid peroxida- tion and scavenging free radical.

关 键 词：心肺复苏 硫化氢 MORRIS水迷宫 学习记忆 

分 类 号：R285.5[医药卫生—中药学]