复方丹参滴丸联合阿托伐他汀对经皮冠状动脉腔内血管成形术后内膜增生的影响  

Effect of compound Danshen dripping pills combined with atorvastatin on restenosis after angioplasty in rabbits

作　　者：宋杰丽 曾锦培[1] 张永霞[2] 黎鹏飞[2] 张利洪[2] 陈次滨[2] 

机构地区：[1]南方医科大学第五附属医院心内科,广东广州510900 [2]广州医科大学附属第三医院心内科,广东广州510150

出　　处：《南方医科大学学报》2014年第9期1337-1341,共5页Journal of Southern Medical University

基　　金：广东省卫生厅科技计划项目(wstjj20121110410203195904221527)

摘　　要：目的探讨复方丹参滴丸和阿托伐他汀联用对经皮冠状动脉腔内血管成形术(percutaneous transluminal coronary angioplasty,PTCA)后内膜增生的影响。方法建立新西兰兔腹主动脉PTCA再狭窄模型,分别采用HE染色和免疫组化技术,观察兔血管内膜增生情况及核因子κB(NF-κB)及单核细胞趋化蛋白-1(MCP-1)的表达活性。结果 HE染色图片结果显示:A组(建模组生理盐水2 ml/kg·d)管腔面积减少、内膜面积增加,内膜增生百分比(%)增高;与A组比较,B组(复方丹参滴丸300 mg/d)、C组(阿托伐他汀2.5 mg/kg·d)和D组(复方丹参滴丸300 mg/d+阿托伐他汀2.5 mg/kg·d)管腔面积增大,内膜面积减小,内膜增生百分比降低,差异均有统计学意义(P<0.05)。D组与B组、C组比较,管腔面积、内膜面积及内膜增生百分比变化更显著,差异有统计学意义(P<0.05)。免疫组化染色显示:B组、C组和D组的NF-kB和MCP-1阳性表达与A组比较明显减少,差异均有统计学意义(P<0.05)。D组与B组、C组比较,NF-kB和MCP-1阳性表达更少,差异有统计学意义(P<0.05)。结论复方丹参滴丸联合阿托伐他汀抑制血管平滑肌增殖的作用优于单用复方丹参滴丸或阿托伐他汀,可能与抑制调节因子NF-κB来降低MCP-1的表达有关。Objective To study the effect of compound Danshen dripping pills and atorvastatin on restenosis after abdominal aorta angioplasty in rabbits. Methods Rabbit models of abdominal aorta restenosis after angioplasty were established and treated with saline （group A）, compound Danshen dripping pills （group B）, atorvastatin （group C）, or compound Danshen dripping pills plus atorvastatin （group D）. HE staining was used to determine the thickness of arterial intimal hyperplasia and assess the morphological changes of the narrowed artery. Immunohistochemistry was employed to detect the expression of nuclear factor-kB （NF-kB） and monocyte chemoattractant protein-1 （MCP-1）. Results Compared with group A, the 3 treatment groups showed significant increased vascular cavity area and reduced intimal area and percentage of intimal hyperplasia （P〈 0.05）. The vascular cavity area, intimal area and percentage of intimal hyperplasia levels differed significantly between group D and groups B and C （P〈0.05）. Immunohistochemistry showed a significant reduction of the expression rate of NF-~cB and MCP-1 in the 3 treatment groups compared with group A （P〈0.05）, and the reduction was especially obvious in group D （P〈 0.05）. Conclusion Compound danshen dripping pills combined with atorvastatin produces better effects than the drugs used alone in inhibiting vascular smooth muscle cell proliferation in rabbits after abdominal aorta angioplasty possibly due to a decreased expression of MCP-1 as a result of NF-kB inhibition.

关 键 词：复方丹参滴丸 阿托伐他汀 血管成形术再狭窄 NF-KB MCP-1 

分 类 号：R541.4[医药卫生—心血管疾病]