视网膜静脉阻塞兔眼微循环变化的激光散斑成像技术定量监测  

Monitoring of retinal microcirculation in rabbit by laser speckle imaging technique

作　　者：吴建国[1] 郑玉强[1] 徐辉[2] 孙静[1] 何伟[1] 

机构地区：[1] 辽宁何氏医学院何氏眼科医院,沈阳,110034 [2]辽宁中医药大学附属医院康复中心脑病科

出　　处：《中华眼底病杂志》2014年第5期492-494,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的 观察视网膜分支静脉阻塞（BRVO）模型兔眼视网膜微循环变化,评估激光散斑成像（LSI）技术定量监测兔眼视网膜微循环的可行性.方法 4月龄健康青紫蓝兔10只纳入实验,选取右眼作为实验眼.裂隙灯显微镜下选择兔眼视网膜毗邻视盘0.5～1.0 mm处的视网膜主干静脉为靶血管,利用仪器自带软件测量靶血管直径以及靶血管0.2 mm^2区域面积的血流量.采用光动力疗法诱导建立兔眼BRVO模型.建模10 min后采用与建模前相同的方法测量兔眼眼底相同位置血管直径和相同区域面积的血流量.结果 LSI技术可获得同步显示的彩色眼底、红外激光和血流分布伪彩图图像.建模前与建模后10 min,靶血管直径分别为（0.104±0.009）、（0.122±0.008） mm,靶血管0.2 mm^2区域面积的血流量分别为（578.400±25.638）、（244.000±49.295）PU.建模后靶血管直径较建模前明显增粗,差异有统计学意义（t=12.114,P=0.008）.建模后靶血管0.2 mm2区域面积的血流量较建模前明显降低,差异也有统计学意义（t=183.00,P=0.009）.结论 BRVO模型兔眼眼底靶血管直径明显增粗,靶血管0.2 mm2区域面积的血流量明显降低.LSI技术可实时定量检测兔眼视网膜微循环状态.Objective To observe the retinal microcirculation changes in chinchilla rabbit with branch retinal vein occlusion （BRVO）,and to evaluate the feasibility of laser speckle imaging （LSI） technology as monitoring tool for retinal microcirculation.Methods Ten 4-month-old chinchilla rabbits were used for the experiment,selecting the right eye as the experimental eye.The main retinal vein,adjacent 0.5-1.0 mm to the optic of rabbit retina,was selected to the target vessel under surgical microscope.The software of LSI instrument was used to measure the diameter of target vein and blood flow of 0.2 mm^2 area of target vein.The BRVO rabbit model was induced by photodynamic therapy,then measure the diameter and blood flow in the same region using the method as before and after 10 minutes modeled.Results The retinal color pictures,infrared laser and the distribution of blood flow pseudo-color were synchronous displayed by LSI technology.Before and after modeling,the target vessel diameter were （0.104±0.009）,（0.128 ±0.008） mm,and the 0.2 mm^2 area blood flow of target vessel were （563.500±28.788）,（256.000±53.319） PU.The diameter of target blood vessel after modeling was significantly thicker than before,with the significant difference （t=12.14,P=0.008）.The blood flow in 0.2 mm^2 area of target vessel was significantly lower than before,also with the significant difference （t =183.00,P =0.009）.Conclusions The diameter of target vessel of the BRVO rabbit model is enlarged,and the target vessel area of 0.2 mm2 blood flow is reduced significantly.LSI system can monitor the retinal microcirculation real-time and quantitatively.

关 键 词：视网膜静脉闭塞/病理生理学 微循环 视网膜血管/病理生理学 

分 类 号：R774.1[医药卫生—眼科]