上皮间质转化相关基因在口腔鳞癌中的相关性及其与临床病理特征的关系研究  

Relations of epithelial-mesenchymal transition-related gene and their correlations with clinicopathological features in oral squamous cell carcinoma

作　　者：赵志娟[1] 袁宏伟[2,3] 韦玄[4] 孙红[5] 云芬 贾永峰[2,3] 施琳[2,3] 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010059 [2]内蒙古医科大学基础医学院病理教研室,内蒙古呼和浩特010059 [3]内蒙古医科大学附属医院病理科,内蒙古呼和浩特010059 [4]中国药科大学药学院,江苏南京210000 [5]内蒙古医科大学基础医学院生物医学综合实验中心,内蒙古呼和浩特010059

出　　处：《临床口腔医学杂志》2014年第9期515-519,共5页Journal of Clinical Stomatology

基　　金：教育部春晖计划合作项目(Z2012007);内蒙古自治区自然科学基金项目(2013MS1132);内蒙古自治区卫生和计划生育委员会医疗卫生科研计划项目(201301038)

摘　　要：目的:观察上皮标志物和转录因子之间的相关性,从而确定这些因子在肿瘤上皮-间质转化(EMT)过程中所起的作用,进一步研究其与口腔鳞癌临床病理之间的关系,揭示其在口腔鳞癌发生发展中的作用及临床意义。方法:免疫组化SP法检测57例口腔鳞癌组织和10例正常口腔黏膜组织中E-cad、Snail、Slug、MMP-2、MMP-9表达,分析其与口腔鳞癌临床病理的关系及E-cad和Snail、Slug、MMP-2、MMP-9表达的相关性。结果:1.口腔鳞癌中E-cad、Snail、Slug、MMP-2、MMP-9的阳性表达率分别为29.82%、75.43%、82.46%、71.93%、78.95%。2.口腔鳞癌中E-cad的表达与分化程度有关(P<0.05),Snail、Slug、MMP-2、MMP-9的表达与分化程度无关(P>0.05);3口腔鳞癌中Ecad、Slug、MMP-2、MMP-9的表达与浸润和淋巴结转移有关(P<0.05),Snail与淋巴结转移和浸润无关(P>0.05)。4.口腔鳞癌中E-cad的表达与Snail、Slug、MMP-9表达均呈负相关(P<0.05),而与MMP-2的表达无关(P>0.05)。结论:转录因子与上皮标志物之间相关性明显,它们与口腔鳞癌的侵袭、淋巴结转移和分化程度相关。这些因子在EMT过程中不同阶段起作用,它们之间的相互作用是口腔鳞癌发生发展的重要环节。Objective： To study the relationship of epithelial markers and transcription factors and confirm their functions in OSCC EMT and investigate their relationship between these factors and clinicopathologic features of OSCC in order to further reveal their roles during OSCC progression. Method： SP immunohistochemical method was used to detect the expression of E-cad, Snail, Slug, MMP-2 and MMP-9 expression in 57 cases of OSCC and 10 cases of normal oral mucosa, analyze their relationship with clinicopathological factors of OSCC and correlation between E-cad and Snail, Slug, MMP-2, MMP-9 expression. Result： （1）.In OSCC tissues,the positive rate of E-cad, Snail, Slug, MMP-2, MMP-9 respectively were 29.82 %, 75.43 %, 82.46 %, 71.93 %, 78.95 % .2 （1） Expression of E-cad was related to differentiation degree of OSCC （P 〈0.05）, but Snail, Slug, MMP-2, MMP-9 were not （P〉0.05） ： （2）Expression of E-cad, Slug, MMP-2, MMP-9 had significantly positively correlated with invasion and lymph node metastasis （P 〈0.05）, Snail has nothing to do with the invasion and lymph node metastasis （P 〉0.05）. 3 The expression between E-cad and Snail, Slug, MMP-9 expression was negatively correlated in OSCC （P 〈0.05）, but not related to MMP-2 （P 〉0.05）. Conclusion： Transcription factors and epithelial markers were correlated significantly, they were related to the invasion and lymph node metastasis and differentiation of oral squamous cell carcinoma. These factors play a role in the different stages of EMT process, the interaction of them is an important part of the development of OSCC.

关 键 词：口腔鳞癌 EMT E—cad SNAIL SLUG MMP-2 MMP-9 浸润转移 

分 类 号：R739.8[医药卫生—肿瘤]