应用二代测序技术筛选压力作用下肝星状细胞的差异表达基因  

Identification of differential gene expression profile of HSCs subjected to pressurization by next-generation sequencing

作　　者：齐峰[1] 胡江峰[1] 唐丹[1] 刘保海[1] 朱樑[1] 

机构地区：[1]中国人民解放军第二军医大学附属长征医院消化内科,上海市200003

出　　处：《世界华人消化杂志》2014年第23期3380-3387,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.11272342~~

摘　　要：目的:应用基因二代测序技术检测肝星状细胞(hepatic stellate cell,HSC)在压力作用后基因表达谱改变及可能机制.方法:分离培养原代肝星状细胞,培养14 d加载10 mmHg的压力1 h,提取总RNA,逆转录cDNA,用DEG-Seq对实验组与对照组进行比较,筛选得到差异基因.结果:肝星状细胞中979条基因出现差异表达,其中14条基因表达差异最为显著,10条基因表达上调,4条基因表达下调,这些基因功能与合成代谢、免疫应答、细胞凋亡等相关.结论:应用基因二代测序技术筛选出压力作用下肝星状细胞差异表达的基因,其可能通过调控这些基因来适应外界压力刺激.AIM： To identify the gene expression profile of hepatic stellate cells（HSCs） subjected to pres-surization by next-generation sequencing.METHODS： Primary HSCs were isolated and cul-tured.After cultivation for 14 d,cells were sub-jected to a pressure of 10 MMHG（millimeters of mercury） for 1 h.Total RNA was then extracted and reverse transcribed into cDNA to screen dif-ferential genes between HSCs subjected to pres-surization and non-treated HSCs using DEG-Seq.RESULTS： A total of 979 differentially ex-pressed genes were expressed in HSCs,of which 14 showed the most significant differ-ence,including 10 up-regulated and 4 down-regulated genes.These genes were found to be related to anabolism,immune response,cell apoptosis,etc.CONCLUSION： Differentially expressed genes could be identified in HSCs subjected to pressur-ization,indicating that HSCs respond to external pressure stimulation by regulating the expres-sion of these genes.

关 键 词：压力 肝星状细胞 肝纤维化 基因二代测序 

分 类 号：R575.21[医药卫生—消化系统]