VEGFR-3 siRNA腺病毒载体对结肠癌细胞生长和运动能力的影响  

SiRNA-mediated silencing of VEGFR-3 reduces proliferation,adhesion and migration of colorectal cancer cells

作　　者：苏芝兰 高峰[2] 吕志诚[2] 张鑫[2] 

机构地区：[1]甘肃武威市医院消化科,甘肃省武威市733000 [2]中国人民解放军兰州军区兰州总医院肛肠外科,甘肃省兰州市730050

出　　处：《世界华人消化杂志》2014年第24期3573-3579,共7页World Chinese Journal of Digestology

基　　金：甘肃省对外合作项目基金资助项目;No.1104WCGA190~~

摘　　要：目的:探讨血管内皮生长因子受体3(vascularendothelial growth factor receptor-3,VEGFR-3)siRNA腺病毒载体对结肠癌细胞增殖、迁移、黏附和侵袭能力的影响.方法:将VEGFR-3 siRNA腺病毒转染结肠癌LoVo细胞,以qRT-PCR和Western blot分别检测VEGFR-3 mRNA和蛋白的表达,通过MTT比色法、划痕实验、Transwell实验、黏附实验检测LoVo细胞的增殖、迁移、侵袭和黏附能力.结果:与空白对照组和阴性对照组相比,实验组VEGFR-3 mRNA和蛋白的表达水平明显降低(P<0.05).与空白对照组和阴性对照组相比,实验组细胞增殖明显抑制(P<0.05),迁移能力减弱(P<0.05),实验组的穿膜细胞数明显减少(P<0.05),黏附能力减弱(P<0.001).结论:腺病毒介导的VEGFR-3 siRNA可以通过下调结肠癌细胞VEGFR-3的表达从而抑制结肠癌细胞的增殖、黏附、侵袭和迁移能力.AIM： To investigate the effect of transfectionwith an adenovirus vector expressing shortinterfering RNA（siRNA） targeting vascularendothelial growth factor receptor-3（VEGFR-3）on cell proliferation, adhesion and migration incolorectal cancer cell line LoVo. METHODS： An adenovirus vector expressingsiRNA targeting VEGFR-3 was constructed andtransfected into LoVo cells. The expression ofVEGFR-3 was detected by RT-PCR and West-ern blot. Cell proliferation and migration weredetected by MTT assay and Transwell assay,respectively. RESULTS： The expression of VEGFR-3 mRNA and protein was significantly decreased after transfection with the recombinant adenovirus（P〈0.05）, compared with the blank control group and negative control group. The proliferation, adhesion and migration of LoVo cells were sig-nificantly decreased after transfection with the recombinant adenovirus（P〈0.05）. CONCLUSION： SiRNA-mediated silencing of VEGFR-3 inhibits the proliferation, adhesion and migration of LoVo cells.

关 键 词：结肠肿瘤 RNA干扰 血管内皮生长因子受体3 腺病毒 增殖 迁移 黏附 

分 类 号：R735.3[医药卫生—肿瘤]