白介素-10基因启动子多态性及其血浆水平与腹泻型肠易激综合征重叠功能性消化不良的关系  

Relationship between IL-10 promoter gene polymorphisms and plasma level and susceptibility to overlap syndrome of diarrhea irritable bowel syndrome and functional dyspepsia

作　　者：黄瑶[1,2] 赵梁[1] 张瑜[1] 周乐 卜平[1] 

机构地区：[1]扬州大学医学院消化病研究室,江苏省扬州市225001 [2]扬州市疾病预防控制中心检验科,江苏省扬州市225001

出　　处：《世界华人消化杂志》2014年第24期3580-3586,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81173392~~

摘　　要：目的:探讨腹泻型肠易激综合征(diarrheairritable bowel syndrome,D-IBS)重叠功能性消化不良(functional dyspepsia,FD)患者白介素-10(interleukin-10,IL-10)基因启动子区域多态性及其血浆水平与疾病易感性的关系.方法:对164例D-IBS重叠FD患者和200例健康对照者抽血提取其白细胞DNA,应用聚合酶反应-限制性片段多态性(polymerasechain reaction-restriction fragment lengthpolymorphism,PCR-RFLP)分析方法以及扩增受阻突变系统-聚合酶链反应(amplificationrefractory mutation system-polymerase chainreactions,ARMS-PCR)结合DNA测序法,对IL-10基因启动子区-592与-1082位点进行多态性分析,并使用酶联免疫吸附法测定血浆IL-10水平.结果:D-IBS重叠FD病例组与正常对照组相比,IL-10启动子区域592位点基因型分布与等位基因频率无显著差异,592CC基因频率显著下降,基因型频率风险相关分析表示,AA基因型是CC基因型患病风险的1.989倍;A等位基因携带型(AA+AC基因型)为CC型纯合子的1.808倍;-1082位点基因型分布与等位基因频率均无显著差异;-592位点与-1082位点组合基因型分布与等位基因频率也无显著差异;不同IL-10分泌水平基因型分布无显著差异.D-IBS重叠FD病例组血浆IL-10平均水平为14.60 pg/mL±7.14 pg/mL,对照组为22.86 pg/mL±10.62 pg/mL,两组比较,病例组显著低于正常对照组(P<0.001).D-IBS重叠FD病例组592位点AA、AC、CC、AA+AC基因型血浆IL-10水平分别为14.50 pg/mL±6.37 pg/mL、14.85 pg/mL±7.75 pg/mL、13.92 pg/mL±7.63 pg/mL,14.68 pg/mL±7.10 pg/mL,病例组各基因型之间血浆IL-10水平无显著差异,592位点AA+AC与CC基因型血浆IL-10水平也无显著差异.结论:IL-10-592位点A和IL-10血浆水平降低可能与罹患D-IBS重叠FD的相关,但这二者之间并无关联.AIM： To investigate the relationship betweeninterleukin-10（IL-10） promoter gene polymor-phisms and plasma levels and susceptibility tooverlap syndrome of diarrhea irritable bowelsyndrome（D-IBS） and functional dyspepsia（FD）.METHODS： Blood samples were collected from164 patients and 200 healthy controls and ge-nomic DNA was extracted. Polymerase chain reaction-restriction fragment length polymor-phism（PCR-RFLP） was used to determine the genotypes in the-592A/C site, and amplification refractory mutation system polymerase chain re-actions（ARMS-PCR） and DNA sequencing were performed to detect the polymorphism of the-1082G/A site. IL-10 plasma levels were deter-mined by enzyme-linked immunosorbent assay（ELISA）. RESULTS： The-592A/C site in the IL-10 gene showed no significant differences in genotypes or allele frequency between the D-IBS-FD over-lap syndrome group and control group, but the frequency of-592 CC genotype was significant-ly decreased in the D-IBS-FD overlap syndrome group. Compared with the CC genotype, the AA genotype and the AA＋AC genotypes in-creased the risk of D-IBS-FD overlap syndrome 1.989 and 1.808 times, respectively. There were no significant differences in the distribution or the allele frequency of-1082 genotypes, the genotypes of the combination of-592 and-1082, and the distribution of the genotypes of differ-ent IL-10 levels between the D-IBS-FD overlap syndrome and healthy control groups. The average plasma IL-10 levels were significantly lower in patients with D-IBS-FD overlap syn-drome than in healthy controls（14.60 pg/mL ± 7.14 pg/mL vs 22.86 pg/mL ± 10.62 pg/mL, P 0.001）. Among patients with D-IBS-FD overlap syndrome, the average plasma IL-10 levels in patients with-592 AA,-592 AC,-592 CC, and-592 AA＋AC genotypes were 14.50 pg/mL ± 6.37 pg/mL, 14.85 pg/mL ± 7.75 pg/mL, 13.92 pg/mL ± 7.63 pg/mL, 14.68 pg/mL ± 7.10 pg/mL, respectively, which showed no significant differences. Plasma IL-10 levels showed no significant difference between the carri

关 键 词：腹泻型肠易激综合征重叠功能性消化不良 白介素-10 基因多态性 启动子区域 

分 类 号：R574[医药卫生—消化系统]