雌激素对人胃癌细胞株PI9表达和细胞增殖的影响  

Effect of estrogen on expression of PI9 and cell proliferation in human gastric cancer cell lines

作　　者：陈海霞[1] 周业江[2] 王璐璐[1] 李华[1] 熊玉霞[1] 

机构地区：[1]泸州医学院药学院药理教研室,四川省泸州市646000 [2]泸州医学院附属医院普外科,四川省泸州市646000

出　　处：《世界华人消化杂志》2014年第24期3613-3618,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30672058;泸州医学院科研基金资助项目;No.2009016~~

摘　　要：目的:观察四株胃癌细胞上雌激素受体(ERα和ERβ)的表达情况及雌激素对胃癌细胞株细胞增殖和丝氨酸蛋白酶抑制蛋白9(serine proteinase inhibitor 9,PI9)表达的影响.方法:对前期筛选出的PI9阳性表达胃癌细胞株(MNK45、SGC7901)和阴性表达胃癌细胞株(MNK28、BGC823),采用免疫荧光化学法检测ERα和ERβ的表达情况,CCK8法检测雌激素干预后胃癌细胞株的增殖情况,逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)检测雌激素干预后PI9表达的变化.结果:ERα在MNK45和SGC7901的细胞核上均有表达,ERβ在BGC823的细胞核上呈弱阳性表达,MNK28上未见表达;雌激素在1-100 nmol/L时可明显促进MNK45、SGC7901的细胞增殖,与阴性对照组相比差异有统计学意义(P<0.05),在10-100 nmol/L时亦促进BGC823的细胞增殖,与阴性对照组相比差异有统计学意义(P<0.05),在10 nmol/L时促进MNK28的细胞增殖;雌激素干预后SGC-7901和MNK45的PI9 mRNA表达均增强,其中,MNK45的PI9 mRNA的相对灰度值(0.7158±0.0734)较对照组(0.5401±0.0653)明显升高,差异有统计学意义(P=0.014).结论:雌激素与胃癌细胞上雌激素受体结合后能明显促进胃癌细胞株的增殖活性,其促增殖作用与其诱导PI9的表达密切相关.AIM： To detect the expression of estrogen recep-tors（ER alpha and ER beta） in four gastric can-cer cell lines and evaluate the effect of estrogenon cell proliferation and expression of serineproteinase inhibitor 9（PI9） in gastric cancer cells.METHODS： Gastric cancer cell lines with PI9 positive expression（MNK45 and SGC7901） andthose with negative expression（MNK28 andBGC823） were used. The expression of ER wasdetected by immunofluorescence. Cell prolifera-tion was tested by CCK8 assay, and expression of PI9 was detected by reverse transcription-polymerase chain reaction（RT-PCR）. RESULTS： ER-α protein expression was noted in MNK45 and SGC7901 cells, ER-β expres-sion was noted in BGC823 cells, and ER-α and ER-β were undetectable in MNK28 cells. Com-pared with the negative control group, estrogen could significantly promote the proliferation of MNK45 and SGC7901 cells at concentrations of 1-100 nmol/L, of BGC823 cells at concentrations of 10-100 nmol/L, and of MNK28 at a concentra-tion of 10 nmol/L（P〈0.05）. The expression of PI9 mRNA in SGC-7901 and MNK45 cells was enhanced after intervention with estrogen, and the relative grey value of PI9 mRNA in MNK45 cells（0.7158 ± 0.0734） increased significantly compared with the control group（0.5401 ± 0.0653, P = 0.014）.CONCLUSION： Estrogen could significantly promote the proliferation of gastric cancer cells by binding to ER, possibly via inducing the ex-pression of PI9.

关 键 词：胃癌细胞 雌激素 雌激素受体 丝氨酸蛋白酶抑制剂9 

分 类 号：R735.2[医药卫生—肿瘤]