胃癌组织中RNF180与RECK的表达水平与预后的关系  

Expression of RNF180 and RECK in gastric carcinoma

作　　者：黄靓[1] 李国庆[1] 毛振江[1] 钟鹰[2] 

机构地区：[1]南华大学附属第二医院消化内科,湖南省衡阳市421001 [2]南华大学附属湘潭医院消化内科,湖南省湘潭市411100

出　　处：《世界华人消化杂志》2014年第24期3660-3664,共5页World Chinese Journal of Digestology

摘　　要：目的:分析胃癌组织中环指蛋白180(the ring finger protein,RNF180)与伴有Kazal域的富含半胱氨酸的逆转诱导蛋白(reversioninducing cysteine-rich protein with Kazal motif,RECK)基因的表达水平及与预后的关系.方法:应用Western blot及逆转录PCR(reverse transcription-PCR,RT-PCR)方法检测胃癌、癌旁组织及其正常胃黏膜中RNF180及RECK的表达水平,分析其表达水平变化与胃癌发生发展的关系.结果:RNF180在胃癌组织及癌旁组织中的表达水平较正常胃黏膜中显著下调(68.7%vs 6.1%,28.4%vs 6.1%,P<0.05);RECK在胃癌组织及癌旁组织中的表达显著低于正常胃黏膜(71.4%vs 5.3%,31.6%vs 5.3%,P<0.05).结论:RNF180及RECK在胃癌组织中表达水平显著降低,提示其可能在胃癌的发生发展过程中有重要作用.AIM： To detect the expression of ring finger protein 180（RNF180） and reversion-inducing-cysteine-rich protein with Kazal motifs（RECK） in gastric carcinoma. METHODS： Western blot and reverse tran-scription-polymerase chain reaction（RT-PCR） were used to detect the expression of RECK and RNF180 in gastric cancer, pericarcinomatous tis-sue and normal gastric mucosal tissue. RESULTS： The positive rates of RNF180 expres-sion in gastric cancer and pericarcinomatous tis-sue were significantly lower than that in normal gastric mucosal tissue（68.7%, 28.4% 6.1%, P〈0.05）. RECK expression was significantly lowerin gastric carcinoma and pericarcinomatous tis-sue than in normal gastric mucosal tissue（71.4%, 31.6% vs 5.3%, P〈0.05）. CONCLUSION： RNF180 and RECK expression in gastric cancer is significantly reduced, sug-gesting that they may play an important role in the development of gastric cancer.

关 键 词：胃癌 癌旁组织 环指蛋白180 伴有Kazal域的富含半胱氨酸的逆转诱导蛋白 

分 类 号：R735.2[医药卫生—肿瘤]