酿酒酵母对特定底物脱卤机制的研究  

Research status summary of halohydrin dehalogenase

作　　者：焦安浩[1] 张松[1] 

机构地区：[1]齐鲁工业大学食品与生物工程学院,山东济南250353

出　　处：《齐鲁工业大学学报》2014年第2期40-42,共3页Journal of Qilu University of Technology

基　　金：山东省博士后创新项目(200903077)

摘　　要：酿酒酵母能够还原2-氯乙酰乙酸乙酯中的顺式和反式的羰基酯。在手性的还原过程中能够进行脱卤的酶对乙酰乙酸乙酯的还原竞争性由细胞质中的谷胱甘肽进行调节。脱氯是由谷胱甘肽体系中尚未确定的谷胱甘肽产生的脱卤酶催化的。第一步反应是谷胱甘肽亲和取代来取代氯原子,然后另一个谷胱甘肽产生脱卤酶来催化硫醚键的断裂。是两个电子转移到细胞质中形成谷胱甘肽氧化物。这个反应是由真菌在有氧的情况下催化完成的,也是一个由谷胱甘肽介导的微生物还原脱卤的例子。Saccharomyces cerevisiae is capable of reducing 2-chloro-acetoacetate in cis and trans ester carbonyl group. In the reduction process can be carried in the hand of dehalogenation enzyme reduction of ethyl acetoacetate competitive cytoplasmic glutathione adjusted. Dechlorination is deter mined by the glutathione system,yet produce glutathione enzymatic dehalogenation. The first step of the reaction is glutathione affinity to replace chlorine atoms substituted,then another dehalogenase to produce glutathione disulfide bond cleavage catalyzed. Two electron transfer is formed in the cytoplasm glutathione oxide. This reaction is made in the case of aerobic fungi finished catalyst,is a microorganism of the glutathionemediated reductive dehalogenation examples.

关 键 词：酿酒酵母 2-氯乙酰乙酸乙酯 谷胱甘肽 脱卤酶 

分 类 号：Q939[生物学—微生物学]