产β-甘露聚糖酶菌株的筛选及产酶条件优化的研究  

Research of the screening of strains producing β-mannanase and optimum conditions of producing β-mannanase

作　　者：单修环 刘钇君 刘新利[1] 

机构地区：[1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353

出　　处：《齐鲁工业大学学报》2014年第3期28-31,36,共5页Journal of Qilu University of Technology

基　　金：山东省科技发展计划(2012GSF12107);山东省高等学校科技计划(J12LD06)

摘　　要：本实验用培养基透明圈法从7种芽孢杆菌中筛选到酶活最高的三株芽孢杆菌(枯草芽孢杆菌4.37、高龙枯草芽孢杆菌w-031 3.83、地衣芽孢杆菌3.83)。用测定发酵液酶活的方法进行菌株的复筛,酶活最高的菌株为枯草芽孢杆菌。对该菌产酶条件进行了单因素实验发酵条件的优化,最优产酶条件为:最佳碳源是魔芋粉,含量为4%,最佳有机氮源为酵母浸粉,最佳无机氮源为硝酸钠,最佳产酶时间为36 h。The experiment screened three strains（ Bacillus subtilis 4. 37,Bacillus subtilis Coland w-0313. 83,Bacillus licheniformis 3. 83） with highest enzyme activity from seven kinds of Bacillus with the method of medium transparent circle. The three strains were rescreened by measuring the enzyme activity of the liquid fermentation. As a result the Bacillus has the highest enzyme activity. The fermentation conditions were optimized by single factor experiment and the optimum fermentation conditions were as follows： konjac flour is the best carbon source and the best content is 4%. The best organic nitro gen source and inorganic nitro gen source are yeast extract and sodium nitrate. The best enzyme production time is 36 h.

关 键 词：Β-甘露聚糖酶 菌株筛选 产酶条件 条件优化 

分 类 号：R979.1[医药卫生—药品]