过表达CD99基因对经典型霍奇金淋巴瘤细胞株L428增殖能力的影响及其分子机制  

Effect of overexpression of CD99 gene on proliferation ability of classical Hodgkin lymphoma cell line L428 and its possible molecular mechanism

作　　者：曾辉[1,2] 黄绪群[3] 宋文玲[1,2] 韩蓉[1,2] 程丽萍[1,2] 石威[4] 

机构地区：[1]江汉大学附属第三医院 [2]武汉市黄陂区人民医院肿瘤三科,湖北武汉430300 [3]黄石市中心医院普爱院区肿瘤内科,湖北黄石435001 [4]华中科技大学附属协和医院血液科,湖北武汉430022

出　　处：《海南医学院学报》2014年第11期1469-1471,1474,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金(81302043)~~

摘　　要：目的:研究过表达CD99基因对经典型霍奇金淋巴瘤细胞株L428增殖能力的影响及其分子机制。方法:培养经典型霍奇金淋巴瘤细胞株L428,分为转染空质粒pcDNA3.1的EVC组以及转染pcDNA3.1(+)-CD99质粒的CD99组,采用MTT法检测细胞增殖情况、流式细胞法检测细胞周期、Western-blot方法检测Bcl-6、Cyclin E的蛋白含量。结果:CD99组的MTT值、G0/G1比例、S期比例低于EVC组,G2/M期比例高于对照组,差异具有统计学意义(P<0.05);CD99组的Bcl-6和Cyclin E含量低于EVC组,差异具有统计学意义(P<0.05);MTT值、G0/G1比例、S期比例与Bcl-6、Cyclin E蛋白含量呈正相关关系,G2/M期与Bcl-6、Cyclin E蛋白含量呈负相关关系。结论:过表达CD99能够抑制L428细胞增殖、诱导L428细胞凋亡,且这一作用是通过抑制Bcl-6、Cyclin E的表达来发挥的。Objective.. To study the effect of overexpression of CD99 gene on proliferation ability of classical Hodgkin lymphoma cell line L428 and its molecular mechanism. Methods： Classical Hodgkin lymphoma cell line L428 were cultured and divided into CD99 group transfected with pcDNA3.1 （＋）-CD99 plasmid, EVC group transfected with empty plasmid pcDNA3. 1. Then cell proliferation was detected by MTT method, cell cycle was detected by flow cytometry, protein levels of Bcl-6, CDC25A were detected by Western-blot method. Results： MTT value, G0/G1 stage ratio and S stage ratio of CD99 group were lower than those of EVC group, G2/M stage ratio of CD99 group was higher that of than EVC group, protein content of Bcl- 6, CDC25A of CD99 group were lower than those of EVC group; MTT value, G0/G1 stage ratio and S stage ratio were positively correlated with protein content of Bcl-6, CDC25A, G2/M stage ratio was positively correlated with protein content of Bcl-6, CDC25A. Oonclusionl Overexpression of CD99 can inhibit proliferation of L428 cell and incde apoptosis of L428 cell; this effect is played through inhibiting the expression of Bcl 6, CDC25A.

关 键 词：霍奇金淋巴瘤 CD99基因 增殖 细胞周期 

分 类 号：R733[医药卫生—肿瘤]